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We narrowed to 919 results for: IRE;

Showing: 401 - 440 of 919 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory...production frustrating - a researcher can complete an entire lentiviral production protocol only to find that...widely requested and incredibly useful tools, they require extensive background work from the requesting scientist...Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs...

  2. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...population. The benefit of beads is that they do not require cells from your experiment, and they are non-specific...., CD4, CD8b, CD11b, CD19), are less likely to require an FMO. Markers that define more specific cell ...often used in the later gates, are more likely to require FMOs. Isotypes Isotype controls are negative controls... populations, but others do not. Some journals require isotype controls, while others don’t. However, ...

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing...specific cell type doesn’t always lend itself to direct application of virus. However, the cargo capacity...infect dividing and non-dividing cells and doesn’t require genomic integration to express its cargo, in fact...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... AAV variant that efficiently infects neurons, directs high-level transgene expression, and has retrograde...any genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport...dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry into the cell. This means...inject a recombinase-dependent chemogenetic vector directly into the region where the cell bodies of the projection...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...used. All of these expression control mechanisms require a specific modification of the viral vector for...of interest, for example. Some customization is required, but many viral constructs have already been built...applications. These libraries include sgRNAs targeting the entire genome, overexpression of all human transcription...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

  6. A Deep Dive into BioBuilder

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    Blog Post
    ...students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt...BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks to our guest blogger, Mary Tamer...

  7. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...(FP) require external light activation, you can’t use fluorescence to monitor processes directly affected...luminescent imaging: A luminescent substrate is required for imaging, and it is important to verify that...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...

  8. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...blessed to have an incredibly supportive Board of Directors at Addgene. Our Board helps keep our focus on ...best practices and encouraging us to explore new directions. I would like to thank them for their time, dedication...can afford to do and when. Addgene’s Legal Team acquired permission from relevant plasmid depositors and...proud to be adding viral vector services to our repertoire. Additional Resources on the Addgene Blog ...

  9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...membrane so DNA can be introduced directly into the cell. Microinjection requires the use of a fine needle to... bacterium’s genome through homologous or site directed recombination. Transduction is a common tool used...donor bacterium to a recipient bacterium through direct contact. The donor bacterium contains a DNA sequence...

  10. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ...ancient Chinese game Go). In addition, there was an entire story-telling session organized by Story Collider...in neurobiology (and really in all of biology) require large data sets. Whether these data sets include...or sequences of microbial DNA they all seem to require some mathematical know-how for analysis. If you...from Addgene. While super useful, these vectors require a bit of expertise and can be difficult for individual...

  11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...is not enough on its own to help you find your desired plasmid. In many cases, you will need a screening...you will need to sort through them to find the desired clone. Do your selection, then screen Why add a...what about identifying clones that contain your desired insert? This is where a  screen comes in. Let’s...process. This way, we can confirm the sequence of the entire plasmid. Please note that NGS verification is more...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... paper for publication in a scientific journal requires several considerations. First, it is important...; the reviewer’s name and affiliations, and the entire referee report are permanently attached to the .... If the reviewers suggest that the manuscript requires revisions, the authors can publish a new version...with the journal’s house style and check that all required information is provided. The clearer you write...

  13. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... “make the ask” once you have found someone you admire and want to learn from. Check out Joanne's Reddit...learn. Mentors can be senior to you or peers you admire.  If you see someone who gives a great talk, ask...path you are interested in following? Someone you admire for their insight and way of expressing it in meetings...locally. Your university probably has an alumni directory. Alumni who enter their information in these searchable...

  14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...programmed to bind to essentially any DNA sequence desired. This technology allows you to create a pair of...knock out a gene. One can also exploit homology-directed repair by flooding the cell with a mutant template...to build a DNA-binding domain to recognize any desired 15 base pair sequence in the genome in rapid fashion...to create zinc finger nucleases that will bind desired sequences. TALENs are much more reliable. If you...

  15. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the other hand...hand, are the “arms” of the Y and comprise the entire light chain and a variable and constant region of...to detect a Fab fragment, it either needs to be directly conjugated to a reporter or you need a light-chain...specific conjugated secondary antibody. These requirements limit the flexibility of and demand for Fab ...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    ...different mechanism thought to be associated with bidirectional transcription. In an uninfected cell, dsRNA ...interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted repeats. These ...genomes which can produce dsRNA as a result of bidirectional transcription of their circularized DNA. These...dsDNA molecule, it does so at the expense of the unpaired DNA strand. A ssDNA molecule exists within the...

  17. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ...is tested following each immunization until the desired level of antibodies is reached - typically within...within 2-4 months. After the animal has the desired level of antibodies in its bloodstream, the antibodies...recognize other antibodies) in experiments that require multiple antibodies that each detect a different...Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of that exact...

  18. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...is the output. The input, plasmid copy number, directly influences gene expression. By itself, increasing... the promoter region, which is predicted to be required to decouple gene expression and plasmid number... example, the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

  19. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...In a recent law review article we received some entirely unsolicited praise: “Addgene, for one, is a sterling... 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised to support our growth. Thanks...outgoing Chair) and Dave Root (incoming Chair) and our entire supportive Board, we have a robust committee process...referrals. Our long reaching reputation helps us hire great people from farther away via connections and...

  20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post
    ...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...salaries - in some cases reversing these decisions entirely. At Future of Research, we had been collecting...are going ahead with raises anyway, including the entire faculty of the biochemistry department at the University...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

  21. Working with Nuclear Receptors

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    Blog Post
    ... some 400 molecules that have been shown to be required by one or more NRs for efficient and timely activation...Primary or core coregulators (those that interact directly with NRs) exist in steady-state complexes with...the new version of the NURSA website will feature direct links to listings of available plasmids for our...relationship with Addgene for the benefit of the entire research community. Thank you to our guest bloggers...

  22. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...reporters specially designed for the aims and requirements of in vivo studies. A particularly useful sensor...or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7...various AAV serotypes, researchers can precisely direct the calcium sensor to specific tissues or even ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

  23. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...that the artifactual PSCs commonly observed with direct stimulation are largely absent in the soCoChR-expressing...BBa_J23100) and expresses N-demethylases and other required enzymes, including a glutathione S-transferase... ΔguaB E. coli are missing an essential enzyme required for conversion of xanthine into guanine, and without...choice for FRET assays where a dark acceptor is desired due to its very low quantum efficiency. ShadowY...

  24. How to Prepare for an Industry Interview

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    Blog Post
    ...heard from friends that Scientist positions often required a one-hour presentation about a prior research...manager or recruiter what presentation format they require. Frequently they will ask you to present about ...similar answer in a following interview. Expect to be tired once the interview is complete Industry interviews... interviewers might.  You’ll probably still be required to give a job talk for a remote interview. Fielding...

  25. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post
    ...formation via NHEJ, precise modification with Homology Directed Repair (HDR), CRISPR activation or repression,... edits compared to DNA transfection.  Homology directed repair (HDR) with Nanoblades CRISPR-based HDR ...To utilize HDR, a donor template containing the desired sequence flank by homology arms must be delivered...with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm or...

  26. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...engineering for each loci, CRISPR/Cas systems only require changing the programmable guide RNA. Gene activation... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...

  27. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...be dull and painful to sit through (imagine the entire audience playing Candy Crush or reading email).... interviews. The idea is to ask questions in a directed way to bring out these interesting stories and... happens, but get things back on track if the direction of the interaction is not interesting to the audience...politely but firmly get them to stop talking and redirect the conversation. The person you cut off might...

  28. Degrading DNA with Cascade-Cas3

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    Blog Post
    ... a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing... or just random mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’...Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature Communications...

  29. Pooled Library Amplifications

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    Blog Post
    ...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...determined experimentally. For larger libraries that require hundreds of nanograms of DNA, you will likely need...bacteria will be its own colony, and will not be in direct competition with other colonies containing smaller...

  30. PhD Applications After COVID

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    Blog Post
    ...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...is right for you outside of just the program requirements: by talking to current students about the work...

  31. 3D Printing Meets CRISPR Cas9

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    Blog Post
    ... steel wire into a backbone structure of a protein, based on its atomic coordinates. These wire models...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for BioMolecular Modeling and...

  32. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...students published their work identifying new genes required for stem and progenitor cell maintenance in planarians...Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their work and become...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance in planarians...

  33. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post
    ...screen for IV deliverable AAVs? Typically AAVs are directly injected into the brain, but this tends to limit... (figure 4f) of the tTA inducer virus, but the desired sparsity of labeling is achieved with lose doses...-color labeling of neurons, because it doesn’t require the generation of a transgenic mouse. 2. Cell-... gene vectors for neurons, no transgenic mouse required (see figure 5 and supplemental figure 8 in Chan...

  34. DNA Purification Without a Kit

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    Blog Post
    ...also less accessible due to their expense and requirement for lab equipment. Plus they create plastic waste... discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits for different purposes...nucleic acids are released by dipping the cellulose directly into a PCR buffer, where DNA or RNA can be amplified...This method takes less than 30 minutes and only requires incubating the columns with 1M phosphoric acid...

  35. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ...content-aware suggestion dropdown list will let you jump directly to the type of information you are looking for...straightforward search is now even easier with a direct link to the plasmid page at the bottom of the dropdown... dropdown list: The search bar will suggest direct links to other types of searches including depositing...search for “Melina Fan” and you’ll be able to go directly to a page to see the plasmids deposited by our...

  36. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...description. You check off all the boxes - you have the desired degree, you have mastered the applications they...person. Similar reasoning may also apply to other acquired skills.” It is nice when other people point out... of a time you’ve lead your team in a defined direction in the past. Maybe there was a time you noticed...list all the skills that your current or past job requires day-to-day, and you’ll get a great snapshot of...

  37. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ...binding-dependent expression of the HIS3 gene, which is required for histidine manufacture and therefore growth...of interest, or a defined cDNA library (e.g. the entire proteome of a particular cell type, or a subset...at the same time in order to try and access the entire interactome of a set of proteins at once.  However...gain access to the nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin...

  38. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post
    ... Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up and running...previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the familial...it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops prior... this saying is especially true. If your event requires a lot of set up or cleanup, ask for volunteers...

  39. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post
    ...cleave both strands. Similar to how Cas proteins require a PAM (protospacer adjacent motif) near the target...a different TAM (5′-TTAT-3′). The unique TAM requirements expand the number of genomic sites that can ... and prime editing (Molla et al., 2021), which require fusing Cas proteins with additional enzymes or ...transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation of compact, ...

  40. QC Sequencing Technologies at Addgene

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    Blog Post
    ...strand is synthesized (Figure 1). Sanger sequencing requires four separate reactions, where a fraction of one...in ultra-long read mode), enough to sequence an entire plasmid in one read. Runs can be completed in anywhere...sequencing, as well as the ability to sequence an entire viral genome in one read. We also use Nanopore ...keeping up with the large amount of QC analysis required every week. They are constantly exploring ways...
Showing: 401 - 440 of 919 results