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  3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...

  6. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...

  11. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...

  12. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...

  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

  14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

  15. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455 19 Coelenterazine None Yes Luminous ...Gaussia princeps) 480 20 Coelenterazine None Yes Metridia longa 480 24 Coelenterazine None Yes North American...

  16. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...

  18. How Addgene Creates Content

    Type
    Blog Post
    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

  22. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

  23. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

  24. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you... mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–...

  26. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...

  27. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...

  28. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...

  29. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

  30. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...

  31. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering... science, these tools sometimes have limited distribution. We hope that will change with Addgene’s expansion...

  32. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...gene or in multiple genes, all of which seem to contribute to a particular disease state. You could use ...

  33. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  34. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...

  35. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...

  37. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

  38. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

  40. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  42. dTAG - You're it!

    Type
    Blog Post
    ...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules...

  44. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

  45. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  48. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

  49. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...
Showing: 501 - 550 of 884 results