...applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility...Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas....201900788 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable...://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based...

...Springer Science+Business Media New York 2014Bustin S; Huggett J. qPCR primer design revisited. Biomolecular...Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of Common Housekeeping Genes as....002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application...

...tools.   References and Resources References Pillay, S., et al. "An essential receptor for adeno-associated...2016. 501-507. PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications...

...chain, called a heavy chain antibody (Muyldermans S., 2013). They have the unique distinction of being... Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies...

...References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving the lock to fit the key to...Smith Y (2019) Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://...

...Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with a number of limitations...

...with gRNA 1 to conduct microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed...cells are needed for proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate...

...process the array into functional crRNAs. Pro-Tip Cpf1’s ability to process its own pre-crRNA arrays simplifies...multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays eliminates...

... can improve the specificity of Cas9 derived from S. aureus. This means you can likely apply similar rational...across a variety of genomic sites. Knowledge of Cas9’s dynamic structure leads to the development of HypaCas9...

...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406...

...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...

...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A. thaliana...

...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

...Other Species Human Mouse Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis M. smegmatis Green monkey...136986 Knockout Human Doench 3rd 2 40,964 Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 161769 Inhibition...Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening N/A 1 1,499 MusCK Library 174196...

... Wu Y, Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem Cell . 2013. PubMed ...colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation... Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. J. Vis. Exp . 2012. PubMed . Western blotting...

...Park, K. U., Schwanke, M., Office, E., Schneider, S. R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski...

... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...

...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...

...Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose an empty backbone...Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...