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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 431 results for: %s

Showing: 201 - 220 of 431 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019). Evolutionary...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) The PAM Requirement and Expanding CRISPR Beyond ...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in...Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable...

  3. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun ...Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073/pnas.89.12.5547...: 27730575; PMCID: PMC5270762. Wendler, F., Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid... the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed...

  5. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...doi.org/10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized...PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium...

  6. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida, S., Toyooka, S., & Otsuka, M. (2025). Genomic Differences ...Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome...Ousterout DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D,.... pii: aad5143. PubMed PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research in human...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility...Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas....201900788 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable...://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ...Springer Science+Business Media New York 2014Bustin S; Huggett J. qPCR primer design revisited. Biomolecular...Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of Common Housekeeping Genes as....002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application...

  10. Arf GTPase Family

    Type
    Collection
    ...Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi...

  11. TALEN Guide

    Type
    Collection
    ...from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...

  12. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...tools.   References and Resources References Pillay, S., et al. "An essential receptor for adeno-associated...2016. 501-507. PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...chain, called a heavy chain antibody (Muyldermans S., 2013). They have the unique distinction of being... Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...with gRNA 1 to conduct microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed...cells are needed for proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...process the array into functional crRNAs. Pro-Tip Cpf1’s ability to process its own pre-crRNA arrays simplifies...multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays eliminates...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406...

  20. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...
Showing: 201 - 220 of 431 results