Viral Vectors 101: Viral Vector Elements
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...making!
References and Resources
Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and ...
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How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun ...Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida, S., Toyooka, S., & Otsuka, M. (2025). Genomic Differences ...Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor... -
Plasmids 101: Repressible Promoters
TypeBlog Post...tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073/pnas.89.12.5547...: 27730575; PMCID: PMC5270762. Wendler, F., Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid... the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome...Ousterout DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D,.... pii: aad5143. PubMed PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research in human... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility...Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas....201900788 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable...://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based... -
Deep Dive: qPCR
TypeBlog Post...Springer Science+Business Media New York 2014Bustin S; Huggett J. qPCR primer design revisited. Biomolecular...Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of Common Housekeeping Genes as....002.Teo IA; Choi JW; Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application... -
Antibodies 101: Affinity Reagents
TypeBlog Post...chain, called a heavy chain antibody (Muyldermans S., 2013). They have the unique distinction of being... Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving the lock to fit the key to...Smith Y (2019) Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...tools. References and Resources References Pillay, S., et al. "An essential receptor for adeno-associated...2016. 501-507. PubMed PMID: 26427452. Issa, Shaza S., et al. “Various AAV Serotypes and Their Applications... -
Human Germline Editing Using CRISPR
TypeBlog Post...with gRNA 1 to conduct microinjection experiments in S phase zygotes at 18 hr post fertilization. They observed...cells are needed for proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...process the array into functional crRNAs. Pro-Tip Cpf1’s ability to process its own pre-crRNA arrays simplifies...multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays eliminates... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with a number of limitations... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post... can improve the specificity of Cas9 derived from S. aureus. This means you can likely apply similar rational...across a variety of genomic sites. Knowledge of Cas9’s dynamic structure leads to the development of HypaCas9... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA ...circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi: 10.1073/pnas.61.4.1406... -
Plasmids 101: Protein Expression
TypeBlog Post...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1 Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A. thaliana... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131. Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...