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We narrowed to 636 results for: ESP

Showing: 201 - 220 of 636 results

  1. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...similar levels of fluorescence when bound to DFHBI despite being approximately half the length (Warner et ...and fluoresce in the red and orange channels, respectively. Both Red Broccoli and Orange Broccoli are derived...Broccoli, and share similar properties to Broccoli despite binding to a different small molecule. Notably,...

  2. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...extra wet lab work, this is still a great option, especially if the options outlined above aren’t realistic...reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options... your editing experiment not go to plan? Don’t despair, you have options! If you think delivery of Cas9...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...but simply whether its concentration changes in response to experimental parameters. This is a job for ... Figure 5: An example gel (left) with bands corresponding to points on the plot of protein abundance versus... Indeed, the quantification in panel B is not especially linear. In panel C, two different methods of ...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...it is for some people, especially women), visibility (how to get it, especially if introverted ), and ...fits in the bigger science picture. Scientists especially like to learn new things, so rehash common knowledge...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...release factor 1, the protein responsible for terminating translation in response to the presence of TAG sequences...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...even see different sets of bands on the gel corresponding to each type of multimer present in your sample...dimer band can sometimes overlap with the band corresponding to the nicked or relaxed conformation of the...Uncut DNA (lane 2) has a band at <3 kb, which corresponds to the supercoiled plasmid monomer, and at <6...

  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...connecting international experts and students. In response to such needs, the International Mentorship Program...progress video conferences and had real-time correspondence via e-mail throughout the entire year. In addition...program, evaluated survey results. Participants’ responses to the mid and post-program self-assessment revealed...

  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Create a monoclonal Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

  10. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...defined environmental conditions. These methods are especially powerful when coupled with iterative rounds of... antibodies with verifiable data is critical, especially when sharing with other scientists. Addgene partners...synthetic antibody products and development methods. Despite recent advances, there is still always room to ...

  11. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...position, what will you need? Multiple first or corresponding author publications (published, not in preparation...audiences and if you pay attention to how the audience responds to your presentations, you will become a more ...experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily mentor for ...

  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light...I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants...field of super resolution microscopy. PA-FPs, especially the monomeric, reversible type, are very well...

  13. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...dyes interact with specific receptors is key, especially for G-protein-coupled receptors (GPCRs). Mutations...also vary in their brightness, depending on the respective fluorochrome. Brightness is impacted by both ...between fluorescent and non-fluorescent states in response to a specific stimuli, which leaves only a small...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...infections. Anything that generates an antibody response in the immune system is referred to as an antigen... is expressed at different times of the immune response and initiates different immune cascades. In the...antibody to recognize. This triggers an immune response within the animal, and its B cells will churn ...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...systems, naming the new systems PE4 and PE5, respectively. These systems improved editing efficiency by... RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at the cost of improved...further evolved the Tf1 and M-MLV RT enzymes, respectively, to obtain PEs small enough to deliver with ...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...bacterial phytochrome BphS which produces c-di-GMP in response to far-red light (730 nm). This nucleotide triggers...high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of particular interest...Zhou et al., 2018). pdDronpa dimers dissociate in response to cyan light (500nm). By fusing the two pdDronpa...

  18. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...has been considering a gradual move off X as a response to major platform changes, such as limiting access... and we acknowledge there may be a variety of responses to this decision. However, our decision allows...

  19. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    ...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...occurs within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...
Showing: 201 - 220 of 636 results