...similar levels of fluorescence when bound to DFHBI despite being approximately half the length (Warner et ...and fluoresce in the red and orange channels, respectively. Both Red Broccoli and Orange Broccoli are derived...Broccoli, and share similar properties to Broccoli despite binding to a different small molecule. Notably,...

...it is for some people, especially women), visibility (how to get it, especially if introverted ), and ...fits in the bigger science picture. Scientists especially like to learn new things, so rehash common knowledge...

...release factor 1, the protein responsible for terminating translation in response to the presence of TAG sequences...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

...but simply whether its concentration changes in response to experimental parameters. This is a job for ... Figure 5: An example gel (left) with bands corresponding to points on the plot of protein abundance versus... Indeed, the quantification in panel B is not especially linear. In panel C, two different methods of ...

...extra wet lab work, this is still a great option, especially if the options outlined above aren’t realistic...reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options... your editing experiment not go to plan? Don’t despair, you have options! If you think delivery of Cas9...

...through our in-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied...expression, and can cross blood vessel barriers. It is especially popular for applications targeting liver tissue...., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-...

...even see different sets of bands on the gel corresponding to each type of multimer present in your sample...dimer band can sometimes overlap with the band corresponding to the nicked or relaxed conformation of the...Uncut DNA (lane 2) has a band at <3 kb, which corresponds to the supercoiled plasmid monomer, and at <6...

...connecting international experts and students. In response to such needs, the International Mentorship Program...progress video conferences and had real-time correspondence via e-mail throughout the entire year. In addition...program, evaluated survey results. Participants’ responses to the mid and post-program self-assessment revealed...

...position, what will you need? Multiple first or corresponding author publications (published, not in preparation...audiences and if you pay attention to how the audience responds to your presentations, you will become a more ...experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily mentor for ...

...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

...Create a monoclonal Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...

...infections. Anything that generates an antibody response in the immune system is referred to as an antigen... is expressed at different times of the immune response and initiates different immune cascades. In the...antibody to recognize. This triggers an immune response within the animal, and its B cells will churn ...

...- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light...I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants...field of super resolution microscopy. PA-FPs, especially the monomeric, reversible type, are very well...

...dyes interact with specific receptors is key, especially for G-protein-coupled receptors (GPCRs). Mutations...also vary in their brightness, depending on the respective fluorochrome. Brightness is impacted by both ...between fluorescent and non-fluorescent states in response to a specific stimuli, which leaves only a small...

...systems, naming the new systems PE4 and PE5, respectively. These systems improved editing efficiency by... RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at the cost of improved...further evolved the Tf1 and M-MLV RT enzymes, respectively, to obtain PEs small enough to deliver with ...

... in this post and we disclaim all liability in respect to actions taken or not taken based on any or all...people will have different roles, titles, and responsibilities during their careers - being aware of this...named in the media report (NPR, New York Times, respected online sources) and the media should discuss your...

...bacterial phytochrome BphS which produces c-di-GMP in response to far-red light (730 nm). This nucleotide triggers...high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of particular interest...Zhou et al., 2018). pdDronpa dimers dissociate in response to cyan light (500nm). By fusing the two pdDronpa...

...has been considering a gradual move off X as a response to major platform changes, such as limiting access... and we acknowledge there may be a variety of responses to this decision. However, our decision allows...

...right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported its...hypothesized that GFP-like proteins could be responsible and began studying these marine organisms to ...

...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...occurs within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...