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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 671 results for: FIE

Showing: 201 - 220 of 671 results

  1. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a convenient...simulations and machine learning, they created a classifier that predicts whether a gene was natural or synthetic...synthetic sequences. The researchers then used their classifier to identify synthetic and natural sequences in...

  2. RUBY-Red Siliques

    Type
    Blog Post
    ...CRISPR-Cas9 gene editing, transformation may be identified by resistance to an antibiotic. Successful editing... of RUBY highlight its use in crop plants under field conditions or in sterile conditions where disturbing...therefore, are useful for research, and they are identified by a phenotype caused by the segregating mutation...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...quicker than larger fragments. These fragments are quantified over time using a fluorescent dye that intercalates... into the DNA such that smaller fragments are quantified first before larger fragments. Figure 1...conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming and ...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with... by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized proteins by Western...alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at ...

  5. Science Career Options

    Type
    Blog Post
    ...Editor Textile Technologist/Designer     Fieldwork and traveling Many people are attracted to science...time outdoors and with nature. Experience in the field or in a position that requires travel can show that...into a career, do your research Once you have identified possible careers, the next step is to research...

  6. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    .... They also come from many different scientific fields, from social sciences to biology, mathematics and... to our volunteer list. We then collected and classified their responses, and used that information to...She is currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Research tackling questions in the fields of Cancer, Inflammation and Immunity, as well as various combinations...combination with chemotherapy. To dive deeper into this field read our recent post “Cancer and the Immune System...Binghamton University! Cancer therapeutics The so called field of Immuno-Oncology, a sizzling hot topic, includes...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...Feature Library - SnapGene’s software seamlessly identifies a variety of common plasmid features such as ...sophisticated detection algorithm easily and precisely identifies plasmid features that differ by only a few nucleotides..., & downloadable .gb & .dna files Once you’ve identified a plasmid that you want to learn more about, ...

  10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...fluorescent reporter. Lastly, once a mutant is identified, the PB transposase can easily and seamlessly...overcome these issues the Denham lab developed a modified monomeric red fluorescent protein (RFP), named...Chen M, Kjems J, Febbraro F, Denham M (2018) A Modified Monomeric Red Fluorescent Protein Reporter for...

  12. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore...takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond...Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed by luciferase, releasing... remains popular, other luciferases have been identified in the sea pansy (Renilla reniformis), click ...to be extra bright. Other luciferases have been modified to change the wavelength of light they emit or...

  15. Clear and Accessible Writing

    Type
    Blog Post
    ...how they felt when they read papers outside their field, the answers were primarily negative. Stupid, overwhelmed...highlight the technical details of collecting the purified AAV–that the interfaces between layers are blurred...virions are captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the ...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants...of the enzymes documented that should narrow the field for a candidate Cas for your experiment. Target...search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID, etc.) and you ...

  17. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on... not well characterized RNA motifs. This bait identified a new interaction: binding of the RC2H1 protein...what host proteins interact with Zika’s UTR and identified QKI, an RNA-binding protein that’s highly expressed...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...tested this system in vitro and in vivo. They identified cells from 19 genera across four phyla that have...conjugation in vivo studies. However, they only identified seven genera in in vitro experiments, possibly...Clostridium difficile. As mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ..., blue, and green channels overlaid on a bright-field image. Image from Ma et al, 2016. Using dCas9... the signal to noise ratio of the system, they modified GFP-dCas9 and the gRNA scaffold to enhance complex... with a number of limitations. Since color is specified by PAM sequence, each color requires a different...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...To solve this problem, gRNAs can be chemically modified to resist degradation while still effectively ...crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications...Hendel, A., Bak, R., Clark, J. et al. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in...
Showing: 201 - 220 of 671 results