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We narrowed to 475 results for: FLU

Showing: 201 - 220 of 475 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... In practice, multiple sources of error (red) influence these relationships and must be addressed with...suggest that common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh... that will be measured in your assay. This is influenced by sample concentration as well as by antibody... lane, your loading control won’t be strongly influenced by unexpected variations in any individual protein...

  2. Plasmid Collections

    Type
    Collection
    ...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more....CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...publication can lead to the publication of rare flukes showing positive results when really there are ...things that make it more likely that we’ll find a fluke when we’re simply trying to get our experiments ...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ...most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (...chromatin structure of the target locus may also influence efficiency. However, Quadros et al. saw success...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...understand and engineer cellular processes that are influenced by temporally ordered biochemical events. How...a unique combination of red, blue, and green fluorescent proteins in each state. The three methods of...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

  6. Worm Expression Resources

    Type
    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  7. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...

  8. Lentiviral Prep Service

    Type
    Collection
    ...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

  9. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous...mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot...: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet ...

  10. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...repetitive regions. Fluorophores may also be fused to gRNA sequences to produce fluorescence at a target region...

  11. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    ...challenge. Chromoproteins are a subset of the fluorescent protein family that give off color in ambient...the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources on the... the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...

  12. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... many of the top 10 posts from this year were influenced by COVID-19 and our new cloning grade DNA service...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

  14. Optogenetics AAV Preps

    Type
    Collection
    ...regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive...

  15. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...(FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...via applications such as western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is ...

  17. RUBY-Red Siliques

    Type
    Blog Post
    ...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...

  18. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another solution...Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining. J Histochem...Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents Antibodies...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...secondary antibodies can be used with different fluorescent labels and both targets can be successfully visualized...

  20. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... often fall short with the generation of various flux imbalances. The conventional approach has typically... the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of...strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can...
Showing: 201 - 220 of 475 results