...Green or Taqman for signaling. Sybr Green is a fluorescent dye that works by binding to all double-stranded...while Taqman uses an intercalating dye, with a fluorescent probe and quencher to quantify specific amplicons...materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-rich DNA and the... Sybr Green chemistry. Blue arrows represent fluorescent signaling in the presence of dsDNA. Image courtesy...

..., if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically be used... We screen the wells in groups as they become confluent, taking both early- and late-arising clones, and...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors...

...sequences for several different fluorescent proteins. Visualizing fluorescence can be challenging in plants...because of the auto-fluorescence of chlorophyll, so we looked at newer fluorescent proteins that get around...

...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...

...broad host range plasmids. The properties that influence host range are not fully understood, but scientists...also control copy number and stability, which influence the plasmid’s ability to establish and maintain...Enterobacteriaceae, Gluconacetobacter xylinus, Haemophilus influenzae, Hypomycrobium X, Legionella pneumophila, Methylophilus...xylinus, Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum...

...generally not true. Because of the variations in fluorescent signals, and how the machine reads/interprets...light that “scatters” past the cell, instead of fluorescent markers. There are various types of SSC and FSC...which is comprised of antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These...anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to identify monocytes (upper...

...cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator to... the sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence is present...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA ...

...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth. Overgrown...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...and discard it if the culture has become overly confluent or too old. Ensuring that you are working with...Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on the production...

... In practice, multiple sources of error (red) influence these relationships and must be addressed with...suggest that common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh... that will be measured in your assay. This is influenced by sample concentration as well as by antibody... lane, your loading control won’t be strongly influenced by unexpected variations in any individual protein...

...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...publication can lead to the publication of rare flukes showing positive results when really there are ...things that make it more likely that we’ll find a fluke when we’re simply trying to get our experiments ...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ...most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (...chromatin structure of the target locus may also influence efficiency. However, Quadros et al. saw success...

...understand and engineer cellular processes that are influenced by temporally ordered biochemical events. How...a unique combination of red, blue, and green fluorescent proteins in each state. The three methods of...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

... pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor... pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine... pAAV-hSyn-GRAB_NE1h Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine...

...Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous...mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot...: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet ...

...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ... to tag endogenous loci with epitope tags or fluorescent markers. Future Directions It’s amazing to see...

...challenge. Chromoproteins are a subset of the fluorescent protein family that give off color in ambient...the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources on the... the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...

... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

... many of the top 10 posts from this year were influenced by COVID-19 and our new cloning grade DNA service...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...