...limit what policy can do and, in this way, science has always been related to government and, consequently...face of evidence” (4). Frustration among scientists has been rising as evidence of climate change, the safety...unwanted results (14). Some loss of trust, I fear, has already come to pass. It may only be by advocating... As of April 13th the March for Science homepage has amassed nearly 500,000 ‘likes’, while their website...scientific funding (17). Federal funding for science has been decreasing for the last five years and the first...for Science: The website for the March for Science has the details for the march in Washington as well as...

...abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting can... DNA is complementary to the probe (for example, has the sequence of the mutated gene the researcher is..., two DNA fragments are observed because the DNA has the site where MstII cuts. In a patient with sickle...DNA fragment is observed. Today Southern blotting has been largely replaced by real-time PCR to answer ... large amount of high-quality DNA, real-time PCR has several advantages including easier automation, higher...questions (see below). Importantly, although their role has changed, restriction enzymes are certainly not dead...

...refined to create Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage tracing...example, Brainbow-1.0 expresses RFP when the construct has not undergone recombination. This default state was...neuronal pThy1-Brainbow constructs, Addgene also has two Brainbow adeno-associated viral vectors (AAV)...Drosophila and zebrafish. Brainbow in Drosophila has aided in the mapping of neural circuits, such as ...neuromuscular junction. In zebrafish, the method has become very useful in lineage tracing; “Zebrabow”...Lichtman estimate that Brainbow’s colorful labeling has decreased the mapping time for a given section of...

... first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently expressed...using chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. ...Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...

... safe to assume that anyone reading this article has a smartphone nearby. We tailor these devices to our...and a couple of other nice features. This app also has a great educational section. This section holds the...this MSDS app. The Chemical Safety Data Sheets app has a search function that allows the user to find an...memorized, but the structures are another story. AMINO has been very helpful when I’ve had to explain the properties...

...CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility to treat... applications.” Li’s team noted that CRISPR/Cas9 has been used to generate mutations in a wide range of... expanded organoids. “CRISPR in adult stem cells has huge potential, because the genomes of adult stem...

...for the flow plots discussed in this post, voltage has been adjusted appropriately and compensation performed...baseline expression of a protein while the green (top) has an upregulated expression (shift to the right). The...overlaps with the baseline suggesting this sample has a comparable expression to the baseline/negative ...allow for better interpretation. Priyamvada Prathima has a research background in protein mutagenesis and ...

...modified version of CAG promoter that the Zhang lab has tested in a variety of mammalian cell lines. Cas9...effective in certain cell lines. This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459...that, upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI restriction sites ...

... in a town with a good public school system that has made strong efforts in the last 5 years to incorporate...curriculum. Our Parent Teacher Association (PTA) has been instrumental in supplementing that curriculum...some quick tips to get things going: If your PTA has a general meeting and a means to request funding ...without them! If you live in a school district that has a lot of parents in STEM careers, chances are they...nearly every talk I’ve given in the past 20 years has been aimed at grad students, postdocs, and other ...

...While much of CRISPR research has focused on genome editing, numerous discoveries have been made using...cleave the DNA. Much of the research using dCas9 has focused on transcriptional activation using a fusion...CRISPR-mediated purification of a specific genomic region has also recently been adapted to isolate genomic loci...tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification...for many other CRISPR techniques, this method also has great potential for improvements and adaptations ...

...altering the sequence of the protein. As DNA synthesis has become quite cost-effective it seems easy for scientists...optimize your gene of interest? Codon optimization has become extremely useful in expressing functional ...neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check...which have even been linked to some diseases. It has been reported that 5-10% of human genes contain a...//doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon optimization of Caenorhabditis elegans...

...technology has been studied and used in assays for over half a century; the Addgene blog has an excellent...new aptamer also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium...initially still unstable in eukaryotes, the Jaffrey lab has since developed new RNA-based scaffolds to contain...

...Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors to light. One lesser-known...effector, and type IV with an unknown effector. Type I has subtypes A through G, with different compositions... Thanks to this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable... job — Cas see DNA, Cas chop DNA. Cas3, however, has two jobs, one as a nuclease and one as a helicase...easier to work with than Cas3. Future targets Cas3 has the potential to be incredibly useful in therapeutics...

... animal moving around to explore the environment has a different motivation (and brain areas associated... certain forms of drug addiction. When an animal has been exposed to morphine or other stimulating drugs...involved in locomotor sensitization? Previous research has shown that regions such as the nucleus accumbens ...dark compartment (Howerton at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors... tests.    Forced swim test The forced swim test has been used since the 1970s to test the efficacy of...

...decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools to the ...The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus...limitations of epitope tags and fluorescent proteins, it has been crucial to develop new tracers that enable accurate...are known to be notoriously difficult to label. It has also been shown that dendrites in the stratum oriens... provided better labels than fusions to 3 or more HA tags. 4. High-resolutions microscopy As indicated...

...Addgene's Special Collections). Arguably, oncology has benefited most as cancer cells are amenable to culture...misidentified cell lines. The solution Fortunately it has never been easier to conduct high quality cell culture...identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...at the scene of a crime (every human in the world has a unique STR profile) and can also be used as a paternity...topics. The LGC Group also provides standards and has laboratories to carry our independent quality control...

...firm The path to a career as a Grant Professional has been a long and winding path for many in the field...?  In the past 5-10 years, the Grants Profession has become just that, a profession. A grant professional...and the increasing regulatory process for grants has fostered the demand for experts in the field of grants... find your people Find someone who is working or has worked as a Grant Professional to mentor and coach...includes the social media community GrantChat. She has served in the nonprofit and government sector for...

... in situ. Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes...protein interactions in living cells. Since then FRET has also been used to measure conformational changes,...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...Fluorescence lifetime imaging microscopy method FLIM has been developed more recently and is the most rigorous...emergence of faster measurement systems, this technique has still many good days and good “gigs” ahead...No offense...

...policy. Since early March, the open access publisher has required authors to include a note as to where readers...how much data was necessary and reasonable. PLOS has since clarified their data sharing policy and recently...Who is to say that the initial data-generating lab has efficiently trawled their own data? A distinct (perhaps...many have multiple, complex panels. PLOS, itself, has not finalized its definition of necessary data requirements.... Secondly, science is highly technical and this has a natural, albeit unintentional, tendency to work...

... and polling, Blugene's new virus friend finally has a name! We thank you for your participation in suggesting...play on viral vector terms. And, the name Kendall has a special place in our hearts: our first two offices...