We narrowed to 437 results for: Hal
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TypeBlog Post...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...
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Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does not use...Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi... -
Transferable Skills Guide: Time Management
TypeBlog Post...experiment, but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...Consider talking to people in the lab across the hall or in the same department as well. What do they ... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...Pamela Hines (Senior Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) ...experiments they’re reading about weren’t changed halfway through in order to chase a positive result. Beyond... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann... -
Hot Plasmids: Spring 2025
TypeBlog Post...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical... -
Antibodies 101: Flow Cytometry
TypeBlog Post...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...Creating epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed ...representing a protein super family). To overcome these challenges, we set out to develop a hassle-free, highly ... -
Using AAV for Neuronal Tracing
TypeBlog Post...replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ... -
A Case Study of Addgene Bioinformatics
TypeBlog Post...our pipeline. In it, they break down the unique challenges of our platform and how we were able to address... -
2022 Depositor Week
TypeBlog Post...on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame Thursday: 8 Great Depositor Research Stories... -
#Deckthelab Contest Winners 2017
TypeBlog Post...used tip boxes for a lab supply tree and set a challenge for her lab mates to create the best-recycled ... -
2021 Deck the Lab Winners!
TypeBlog Post... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog Post...Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:45-28:07 : How Seeding...