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We narrowed to 433 results for: Hal

Showing: 201 - 220 of 433 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs’ size also provides...plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...

  10. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

  13. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...

  14. Optogenetics Guide

    Type
    Guide
    ...plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula (Halobacterium...when one half of a given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1...Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni ), causes hyperpolarization (inhibition...enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR...strain Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...

  16. CRISPR Guide

    Type
    Guide
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...

  18. 2022 Depositor Week

    Type
    Blog Post
    ...on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles...
Showing: 201 - 220 of 433 results