We narrowed to 7 results for: Hal

1. Gibson Assembly Protocol

   Type

   Protocol

   ...part, one part has half of the antibiotic gene and the adjacent part has the other half. Any colonies should...

2. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...medium at a reduced antibiotic concentration (e.g., half the concentration that was used for the initial ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...

3. Weighing Reagents Protocol

   Type

   Protocol

   ...Pro-Tip If you’re using weighing paper, fold it in half first to create a crease and then place it unfolded...

4. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...

5. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ... up and down at least 10 times, and use at least half of the final volume (mix with >50 uL if your well...

6. AAV Production in HEK293 Cells

   Type

   Protocol

   ... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

7. CRISPR Library Amplification

   Type

   Protocol

   ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...