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We narrowed to 8 results for: Hal

Showing: 1 - 8 of 8 results

  1. Optogenetics Guide

    Type
    Guide
    ...plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula (Halobacterium...when one half of a given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1...Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni ), causes hyperpolarization (inhibition...enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR...strain Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...

  2. CRISPR Guide

    Type
    Guide
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production Your Lentiviral Plasmid...https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G., Bolandian, M., Dorostkar, R., Jafari....org/10.1016/j.omtm.2020.10.012 PMID: 33768124 Marshall, H. M., Ronen, K., Berry, C., Llano, M., Sutherland...https://doi.org/10.1089/hum.2012.229 PMID: 23311447 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...

  4. Adenovirus Guide

    Type
    Guide
    ...provide the deleted adenoviral genes. The biggest challenge of the HCAdV vector production is to ensure no... protect nonhuman primates against ebolavirus challenge . Journal of Virology, 85 (9), 4222–4233. https...

  5. Molecular Biology Reference

    Type
    Guide
    ...for adding the next nucleotide, the reaction is halted once the fluorescently tagged base is incorporated...converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does not require...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable...) and a gRNA together into an rAAV capsid is challenging due to its limited packaging capacity, especially...

  7. Sequencing Primers

    Type
    Guide
    ... forward primer pCasper-F GGGTTTTATTAACTTACAT (Vosshall lab) 5' end of Drosophila mini-white gene, reverse...

  8. Chemogenetics Guide

    Type
    Guide
    ...Ikefuama EC, Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...
Showing: 1 - 8 of 8 results