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  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...is probably in the thousands! The goal of this article is to provide enough background for you to distinguish...Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ... Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal Gray...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...then, is this: It’s the optimal number of viral particles per volume required to express the gene of interest...spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434-019-0075-6 Issa...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... conformation of the plasmid monomer (see this article from BiteSize Bio for more information on how different...linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene uses Illumina...

  6. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...How many times have you ever seen a scientific article attributed to a single author? I’ve seen so few...years and there are currently about 5.5 million articles in the English version of Wikipedia alone. Humans...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... require the use of potentially harmful viral particles that express oncogenes, such as Myc. Viral vectors...RNAs are serially transfected using a cationic vehicle. The following synthetic modifications are used...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...list of publications we've highlighted in this article.  Publication Plasmids   Highlights Komor...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ...sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different rAbs that ...binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available Indirect...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different...

  12. Lentiviral Guide

    Type
    Guide
    ...plasmids) in order to produce functional lentiviral particles than 3rd generation systems. 2nd generation packaging...plasmids in order to create functional lentiviral particles. IMPORTANT : A 3rd generation transfer plasmid...envelope protein is commonly used in lentiviral particle production because it confers broad tropism over.... For more information, see the Cronin, et al. article on different envelopes and their tropism . How ...cell genome, but cannot produce functional viral particles without the genes encoded in the envelope and ...the resulting construct is packaged into a viral particle, it is not possible for viruses normally produced...Precursor structural protein of the lentiviral particle containing Matrix, Capsid, and Nucleocapsid components...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...adenovirus gene E1+, to produce infectious AAV particles. Rep/Cap and the adenovirus helper genes may also...-CRISPR, please see this blog post. Browse the article from Ran et al. , 2015 from the Zhang Lab to find... frequency. Nonetheless, the majority of rAAV particles are thought to be maintained in episomes or concatemers...concatemers. Episomes differ profoundly from viral particles produced during a lytic cycle. rAAV episomes can...without damaging the host cell. In contrast, viral particles produced during a lytic cycle are quickly released...

  14. Chemogenetics Guide

    Type
    Guide
    ...respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations at L131G, Q139L,...and is termed PSAM4 (or PSAM 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs)...Effect Outcome (in neurons) Reference PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition...inhibition Magnus et al., 2019 PSAM4 5HT3 Varenicline, uPSEM 792, uPSEM 817 Na + , K + influx Neuronal...
Showing: 201 - 220 of 395 results