We narrowed to 396 results for: ICL
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TypeBlog Post...is probably in the thousands! The goal of this article is to provide enough background for you to distinguish...Mammalian Vectors Looking for More Plasmids 101 Articles? Find them all at our Plasmids 101 Topic Page...
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Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...then, is this: It’s the optimal number of viral particles per volume required to express the gene of interest...spinal cord following injury. Gene Therapy, 26(5), Article 5. https://doi.org/10.1038/s41434-019-0075-6 Issa... -
Plasmids 101: Dimers and Multimers
TypeBlog Post... conformation of the plasmid monomer (see this article from BiteSize Bio for more information on how different...linear and nicked plasmids, as described in this article. Long-read sequencing Addgene uses Illumina... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...Biosciences and Technology. If you are reading this article then you probably suspect mycoplasma contamination...the Addgene blog Browse Addgene's viral vectors articles Find tips and tricks for working with viral vectors... -
Transferable Skills Guide: Teamwork
TypeBlog Post...How many times have you ever seen a scientific article attributed to a single author? I’ve seen so few...years and there are currently about 5.5 million articles in the English version of Wikipedia alone. Humans... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... using our TAG-IN bioinformatics tool.A recent article by Clarke et al shows that crRNAs binding to the...for the csRNP method is described in our recent article (Dewari et al. 2018), however, if you have any ... -
Delivery Methods for Generating iPSCs
TypeBlog Post... require the use of potentially harmful viral particles that express oncogenes, such as Myc. Viral vectors...RNAs are serially transfected using a cationic vehicle. The following synthetic modifications are used... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options for CRISPR plasmid...answers on the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse...list of publications we've highlighted in this article. Publication Plasmids Highlights Komor... -
Technical Design of a Western Blot
TypeBlog Post...sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different rAbs that ...binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available Indirect... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog Post...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository ... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog Post...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository ... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog Post...Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository ... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from Addgene. ... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post... preps. For example, last year we published an article describing a sucrose supplementation strategy to... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...started with COVID-19 research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...Kamens. As a regular contributor of careers blog articles, Joanne had plenty of advice for the group of ... -
A Scientist's Guide to Ebola
TypeBlog Post...how the glycoprotein transforms itself to escape vesicles within host cells and turn the cell into a veritable... -
Addgene's Website Update
TypeBlog Post...guide, with links to our most helpful depositing articles. If you’re depositing data, instead of materials...