We narrowed to 298 results for: IRES

1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   Blog Post

   ...editors The most commonly used S. pyogenes Cas9 requires the target site in the genomic DNA to contain ...

2. Engaging with science and society at pgEd

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   ...communication A career in science communication or policy requires broad interest in issues at the interface of science...

3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ...of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline induced expression...

4. Plasmids 101: Modular Cloning

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   ...simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For example...

5. Viral Vectors 101: Calcium Sensors

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   ...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

6. Viral Vectors 101: Chemogenetics

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   ...Targeting Receptor Expression Using chemogenetics requires successfully introducing receptors to target cells...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm or...

8. Science communication: 9 Strategies to Get Your Foot in the Door

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   ...around climate change, biodiversity, or genomes requires lots of data. Get your community involved in sampling...

9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...genome. AAV belongs to the family Parvoviridae and requires co-infection of helper viruses like adenovirus...

10. Antibodies 101: Choosing the Right Isotype

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    ...expressed at very low levels or your application requires a high degree of affinity, then species choice...

11. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...Cas9. Cpf1 cleaves DNA in a staggered pattern and requires only one RNA rather than the two (tracrRNA and...

12. Plasmids 101: Terminators and PolyA signals

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    ...certain RNAs. Unlike the eukaryotic mechanism which requires a consensus sequence for the addition of a poly...

13. Tips for Titering Your Lentiviral Preps

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    .... Developing the proper control line typically requires transducing with a limiting multiplicity of infection...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...proteins). The chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference...

15. Illuminating Epigenetics with A FRET Based Biosensor

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    ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...

16. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...Different leadership styles Being an effective leader requires you to understand how to work with many different...

17. Degrading DNA with Cascade-Cas3

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    ...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...  But llama immunization has its drawbacks: it requires a camelid animal facility which can be expensive...

19. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...a few aspects in which spectral flow cytometry requires special considerations. Conventional flow cytometry...

20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ... were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide sequences...