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We narrowed to 771 results for: LIS

Showing: 201 - 220 of 771 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...endogenous homologous recombination enzymes can also accomplish the desired genomic modification. This technique... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...binding site (using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the...scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise..., Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...get people to remember you). What better way to accomplish all of these things naturally than to give a ... talk without looking at their slides or notes. Listen to their stories and narratives (see below “Tell...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many... Tell a story We are biologically programmed to listen and remember better when we hear material delivered...should be very simple. The goal is to get the listeners to be thinking “What happens next?”  Describe .... During your practice rounds, encourage your listeners to ask questions and record those that were asked...

  3. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ... Video Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies...Hola! Que tal? My name is Brandon Solis, and I am a rising senior studying Sociology and Dance at Skidmore...of challenging what I am used to. Brandon Solis, marketing intern. Something that I struggle ... Brandon Solis...

  4. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...which he submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which... publications, although many don’t necessarily publish in OA journals themselves. A good number of those...

  5. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...is busy and has a To-Do list long enough that there is no hope of accomplishing it all. How do we encourage...teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus on a single... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...wonderful tradition for university departments to establish, with the goal of fostering better communication...

  6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...along the way in this ongoing series of blog posts. Listen to our podcast interview with Harvard Medical School...’ – inspire you.” “Also a mentor can give you realistic expectations. (Don't think you can finish that...is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is Yoda who is... concrete goals and helps you reach your goals Listens well and communicates clearly and openly Gives ...like to be validating without being coddling, to listen without being taken advantage of, and to respect...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to...identifiers and links provided, in addition to a list of individual screens curated for the publication... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... full picture and listen for hints at the same concerns from multiple people. Listen to what they say,...path and help you pursue any career alternative? Listen to our podcast interview with Harvard Medical School...Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use publication records... end of this blog post. This document includes a list of questions to ask your potential advisors and ...

  9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ... a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT: A highly...transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers from... surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies...Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr’s lab... C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast segment Similarly to the...

  10. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting...read, I pay more attention to details and use a checklist for individual sections of the paper (see figures...authors put in the manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge...sharing whatever she knows with whoever cares to listen. In March 2020 she will start her own research ...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent ...example, cell lysate or media from cultured cells. ELISA can be used to quantitatively measure the amount...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

  12. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from...collaborations. Alternatively, perhaps the lab publishes and presents regularly with labs around the world...you might enjoy working closely with others to accomplish your goals. You must understand your own style...for Scientists". This post also includes a useful list of questions for a candidate to ask lab heads, lab... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  13. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly efficient...knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans RNAi podcast segment RNA interference... miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment A ... outbreaks Article contributed by Izzy Mueller  Listen to the viral detection podcast segment As the ... motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment The human proteome...

  14. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...plasmid deposit please sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted...degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi...nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first...epigenome Article contributed by Joanne Kamens Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree... in yeast Article contributed by Tyler J. Ford Listen to the Synthetic Yeast Transcription Factors Podcast...

  15. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...each listed: An “official” name for the container to be used in cross-team communication A list of “AKA...lexicon. The base lexicon was and continues to be a list of commonly used (and misused) words that clearly...the training frequently. In hindsight, getting a list of frequently used words like this when starting...Happier colleagues. Feeling like people aren’t listening to you or understanding you can be incredibly ...

  16. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...scientists”  These comments are well correlated with published data. For example, in 2010, less than 15% of postdocs...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing... Biology at Brandeis University, has recently published an article arguing against reducing the number...postdocs. Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

  17. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode! Expanding the optogenetics...CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment The Won Do Heo...optogenetics Article contributed by Meghan Rego  Listen to the soCoChR podcast segment Optogenetic stimulation...operon Article contributed by Angela Kaczmarczyk  Listen to the synthetic decaffeination operon podcast ...fluorescent proteins for use as FLIM-FRET acceptors  Listen to the FLIM-FRET acceptor podcast segment Article...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...helped me remember them for the interview.  Prepare a list of questions to ask your interviewers You’re interviewing...“questions to ask an interviewer,” you can find lists that have lots of general questions about job responsibilities...before the interview. If you are not provided with a list of who will be interviewing you, ask the hiring ... this information. I found it useful to write my list of questions in a notebook that I took with me to.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...originality to your work is to collaborate with specialists in different fields that can provide different...insight into an area - not just working within the established network of your peers. Furthermore, using new...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...

  20. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo Fisher Scientific...landscape facing all scientists, both young and established, as they embark on or continue their careers ...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor... Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University...
Showing: 201 - 220 of 771 results