We narrowed to 50,992 results for: LIS

1. B. subtilis Single Gene Deletion Library--Kanamycin

   Plasmid Kit

   #1000000115

   Purpose

   B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.

   Depositor

   Carol Gross

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

2. B. subtilis CRISPRi Essential Gene Knockdown Library

   Plasmid Kit

   #1000000116

   Purpose

   B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.

   Depositor

   Carol Gross

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

   Plasmid

   #164611

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S383T

   Promoter

   CMV

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti AID-BEmax

   Plasmid

   #157950

   Purpose

   Lentiviral expression of AID-BEmax

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Lenti AID-BEmax
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-GRAB_ATP1.0

   Plasmid

   #167577

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hsyn-GRAB_5-HT1.0

    Plasmid

    #140552

    Purpose

    Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    5-HT sensor GRAB_5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

    Plasmid

    #111818

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Mef2c (Mef2c Mouse)
    Tdtomato
    Gata4 (Gata4 Mouse)
    Tbx5
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTS-mCherry-GFP1-10-Hyg-N1

    Plasmid

    #91957

    Purpose

    Expresses mCherry-GFP1-10 in mammalian cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    hSyn

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXBI-mclover-YTHDC1-FL

    Plasmid

    #177121

    Purpose

    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCDH-puro-CMV-VC3AI

    Plasmid

    #78907

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    VC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge