Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 25 results for: LIS

Showing: 1 - 20 of 25 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Validation Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may also like...Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs and When to Use Them Immunocytochemistry...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected... an indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...reproducibility in science. General Considerations The ELISA can be completed in a single day or broken up over...done by hand but if available, use an automated ELISA plate washer. Equipment Spectrophotometer compatible...

  2. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Primers for reference). Many sequencing cores have a list of common primers that can be requested for no additional...used by Addgene during quality control? Addgene lists the primers used to obtain each result above the...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

  9. Ligation Independent Cloning

    Type
    Protocol
    ...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

  10. Video Library

    Type
    Protocol
    ...community. Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current...

  11. Centrifugation

    Type
    Protocol
    ...speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often given...

  14. Immunocytochemistry

    Type
    Protocol
    ...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...
Showing: 1 - 20 of 25 results