We narrowed to 527 results for: MEA;

...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...

...each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...

...genomes/mL) can be measured by: Optical density: particle concentration is measured by looking at absorbance...

... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle... (or BSL-2+) lab and require additional safety measures.   General viral handling safety   Containment...

...particles (VLPs). VLPs lack viral genomes which means they are non-infectious and non-replicative. Nanoblades...iPSCs and BM cells after Nanoblade treatment as measured by their maintenance of pluripotency marker expression...

...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...

...this does not mean that you should apply for everything that’s out there, it just means that you shouldn...

...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn...

...do it well" – even the smallest program. I don't mean have fancy food or a fantastic view (these are nice...Timing is everything Many of my suggestions are meant to keep the event on time. Nobody likes a program...

... sum game - that is, if I get what I want, that means you don’t. In this mindset, one of us wins, the ... negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all won...

...Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et...found a way to use the accumulation of indels to measure time. These plasmids with different promoters driving...

... control assays for AAVs. These steps include measuring viral titer and assessing purity. But Addgene ...get hits to “random” genes. Do these hits always mean the sample is contaminated? No - what really matters...

...cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach their intracellular...colocalization experiments in a scientific and meaningful way it is important to choose the appropriate...

...have died, usually after four to five days. This means that viral production takes a little bit longer ...Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production...

...aim for somewhere between 30x and 200x coverage, meaning that you need 30 to 200 times as many colonies ...recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura...

...limit can feel like a lot of pressure. However, it means that your manager (and you) will be motivated to...time, you’ll know more people in industry, which means you’re more likely to have the right contacts and...

...suggest retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work,...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

...nontrivial propensity to noncovalently dimerize. That means that your POI fused to GFP or another fluorescent...whorl formation assay, are practical real world measures of dimerization potential that will determine ...

...vectors do not have to exclusively be used as a means of detection; they are useful almost anytime you...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional...

...Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of antigens (... size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered...