We narrowed to 252 results for: Mac

1. Simplify Cloning with in vivo Assembly

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   ..., but have successfully tested DH5a, TOP10, and Mach1. No common lab cloning strain has failed so far....

2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

3. AAVs in Retinal Gene Therapy

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   ...retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing and some will hopefully...

4. Tips for Screening with Yeast Two Hybrid Systems

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   ...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ...

5. Plasmids 101: Protein tags

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   ...Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D.,...

6. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ... compared with Google Docs and wikis - by Rory Macneil on Bitesize Bio Data Freedom: The Expansion of ...

7. Save Time with Transient Plant Leaf Transformations

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   ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...

8. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   ...and historically, bagpipes were made using the stomachs of different animals. Check out the Addgene website...

9. Hot Plasmids - August 2020

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   ...immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow of ...

10. Finding nucleic acids with SHERLOCK and DETECTR

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    ... Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...

11. New CRISPR Tools: Cas7-11 and PASTE

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    ...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ...

12. Twenty Years of Sharing: Expanding Accessibility

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    ...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...

13. How to Be an Excellent Trainee

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    ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

14. CRISPR-mediated Plant Base Editors

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    ...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

15. Transferable Skills Guide: External Collaborations

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    ...become popular. There are only so many methods and machines one team can learn so linking together expert ...

16. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...2008. 8(3): p. 147-61. PubMed PMID: 18537590. 9. Mack, A.A., et al., Generation of induced pluripotent...

17. Plasmids 101: Gateway Cloning

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    ...coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene is present in the donor vectors ...

18. Getting the Most from Your Lentiviral Transduction

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    ...Addgene's New Viral Service! References 1. Hay, R.J., Macy, M.L. & Chen, T.R. “Mycoplasma infection of cultured...

19. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

20. Plasmids 101: The Promoter Region – Let's Go!

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    ... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...