We narrowed to 5 results for: Mac
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TypeProtocol... .ab1 files, such as 4Peaks (Mac), SnapGene Viewer (Mac/PC), FinchTV (Mac/PC), Sequence Scanner (PC), ...
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AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic...NaCl to destabilize ionic interactions between macromolecules. The 40% and 25% steps are used to remove contaminants...option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... -
DNA Quantification
TypeProtocol...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...