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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 420 results for: Numb

Showing: 201 - 220 of 420 results

  3. Reflections and Looking Ahead

    Type
    Blog Post
    ...commitment to open science and shared progress. But numbers tell only part of the story. This year, we made...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    ...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has huge ...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    ... benefits of depositing… you’d be amazed by the number of out of office responses I’ve received). Clearly...

  10. CRISPR Library Amplification

    Type
    Protocol
    ...100 x 100 x 100 ÷ 0.1 This number should be at least 1000X greater than number of perturbations in library...plasmids and can be scaled according to the actual number. Required Quality Control (QC) Pooled libraries...individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip Use of extreme...order to be enumerated accurately. Frequently the number of colonies can be too great to count. Ideally,...a given plate (example: by increasing the total number of bioassay plates used). Scaling up the experiment...Maxiprep protocol, scale the reagent volume and column number as needed. The use of Mega or Gigapreps is acceptable...acceptable when scaling up or reducing the number of tandem purifications. Consult the manufacturers handbooks...

  20. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...replication (which controls the host range and copy number of the plasmid) and typically include a gene that...
Showing: 201 - 220 of 420 results