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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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  1. Antibody Guide

    Type
    Guide
    ...valency, or number of binding sites available per antibody unit, which is typically the number of antibodies...while the number of proteins that you can detect in a single assay is limited by the number of signaling...therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. Check for and...antibody, which becomes the limiting factor for the number of proteins you can detect in a single assay. Overview...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...denoted using two numbers separated by a slash (e.g. AAV2/5), where the first number indicates the serotype...far been identified, and it is likely that this number will continue to increase. This diversity makes...serotype of the genome and the second number the serotype of the capsid. Pseudotyped vectors can improve... BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that can target whole ...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ... the number of plasmids present in the library - for example, the Moffat lab uses 200x the number of cells...cells as plasmids in the TKO library. Using large numbers of cells minimizes the effects of random chance...

  4. Adenovirus Guide

    Type
    Guide
    ...denoted using two numbers separated by a slash (e.g. Ad5/35), where the first number indicates the serotype...serotype of the genome and the second number the serotype of the capsid. Use of these pseudotyped vectors...

  5. Molecular Biology Reference

    Type
    Guide
    ...constructed a plasmid, you can easily make an endless number of copies of the plasmid using bacteria, which ...BL21 and its derivatives. We've included a small number of E. coli strains below and recommend checking...

  7. Plan Your Experiment

    Type
    Guide
    ... target DNA may result in reduced cleavage. The number of alleles for each gene may vary depending on ...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...in trans ). This method, therefore, reduces the number of plasmids that are required in the transfection...
Showing: 1 - 8 of 8 results