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...valency, or number of binding sites available per antibody unit, which is typically the number of antibodies...while the number of proteins that you can detect in a single assay is limited by the number of signaling...therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used in the lab. Check for and...antibody, which becomes the limiting factor for the number of proteins you can detect in a single assay. Overview...

...Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants of LRRK2...Labs affiliated with MJFF have also generated a number of Shared Research Tools (Link opens in a new window...

...mutant and wild type. The ability to manipulate the number, orientation, and type of target sites that flox...R297T), a point mutant iCre variant, reduces the number of recombination events but can improve specificity...

...for designing optimal gRNAs. Provides feedback on number of potential off-targets, target's genomic location...in an input sequence, which can be an accession number, genomic location, pasted nucleotide sequence, ...

...Looking For? At this time, Addgene has made a limited number of plasmids available as cloning grade DNA that...

... lower the repression level. By controlling the number of mismatches we can obtain a range of expression...

...REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional...

[email protected] and acknowledge NIH grant number R01 GM088040 in the publication. References Rapid...

...Figure 1A ). Scientists have identified a large number of zinc fingers that recognize various nucleotide...

...Kit. These modified pFUS vectors can reduce the number of module plasmids and improve the success rate...

...today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window) available...

...further downstream in the NID and C-terminus. A large number of mutations are known to cause Rett syndrome, ...

...Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads in ...

...within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the ...

... highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification...

...neuroscience research applications. A substantial number of these have been cloned, expressed and validated...