Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 563 results for: PAC

Showing: 201 - 220 of 563 results

  1. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...will likely be spending a few hours on your feet. Pack water and snacks to keep you energized throughout...session, bring extra clips to hold up your poster, and pack a few business cards to tack up next to your poster...hearing the highlights so that you can appreciate the impact of the project as a whole. In addition to time ...

  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...are sharing stories of our repository’s positive impact on science and scientists around the globe. Our...CRISPR plasmids to labs in ~100 countries.  The impact Addgene’s CRISPR collection contained 197 plasmids...citations.  CRISPR is a story that centers around the impact of science, but our data makes it clear that it...

  3. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...committees, and how the culture of the university impacts the number of women reaching senior positions. ... those amazing students that want to have a big impact on the world and tell them to work in plant science...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four proteins...INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations...INTEGRATE system follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    ..., including the E1 region). The space in the viral particle’s capacity that is made available by this ...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  9. Plasmid Collections

    Type
    Collection
    ...Kits Kits are groups of plasmids that have been packaged together because they are intended for use as ...Initiative Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium...

  10. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares), and also...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...26 ideas, over half of which, when implemented, impacted the entire company. It is important to note that...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

  14. Hot Plasmids - November 2023

    Type
    Blog Post
    ...  Here's what you'll find in this post:  New compact, efficient prime editors A versatile and precise...tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna Stroik The ...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983–4002...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive ...Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells allows production...and composition of your library can drastically impact what conditions it needs to properly amplify.  ...

  16. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...routinely use Nanopore technology to sequence our packaged viral DNA for quality control. This gives us a...the issue and resolve the assembly. Keeping up the pace Our QC team constantly faces the challenge of keeping... backend, has enabled the QC to keep up with the pace of deposits while maintaining quality. Maintaining...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...resources: ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their...Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in ...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent ...” gate in the beginning should not have a great impact on your analysis, and I often choose a looser gate...separation pattern largely stays the same and does not impact my ability to draw a proper gate, I am free to ...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...
Showing: 201 - 220 of 563 results