...will likely be spending a few hours on your feet. Pack water and snacks to keep you energized throughout...session, bring extra clips to hold up your poster, and pack a few business cards to tack up next to your poster...hearing the highlights so that you can appreciate the impact of the project as a whole. In addition to time ...

...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...

...a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60 mm dish...the plates that do not appear to be yielding well-spaced clones. For rapidly growing cells we sometimes ...the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences) to...

...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...committees, and how the culture of the university impacts the number of women reaching senior positions. ... those amazing students that want to have a big impact on the world and tell them to work in plant science...

... Viral Vector Packaging Service Viral Production Viral Production Jump...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture...

...top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four proteins...INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations...INTEGRATE system follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo...

...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

...sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006...expressed transiently by transfection or stably impacts the success of BiFC experiments. In either case...//doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of protein homomerization...

..., including the E1 region). The space in the viral particle’s capacity that is made available by this ...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...

...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive ...Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells allows production...and composition of your library can drastically impact what conditions it needs to properly amplify.  ...

...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...26 ideas, over half of which, when implemented, impacted the entire company. It is important to note that...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...  Here's what you'll find in this post:  New compact, efficient prime editors A versatile and precise...tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna Stroik The ...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983–4002...

...resources: ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their...Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in ...

...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

...Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent ...” gate in the beginning should not have a great impact on your analysis, and I often choose a looser gate...separation pattern largely stays the same and does not impact my ability to draw a proper gate, I am free to ...

...techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ from the short-read...1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer reads are able to... Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production. Biotechnol...

...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...