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We narrowed to 538 results for: PAN

Showing: 201 - 220 of 538 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...hypotheses being rejected, etc.), while reminding participants of the value of this approach and encouraging...by making a greater number of students active participants in the research process – so if this sounds ...classroom-based undergraduate research experiences spanning chemical biology, biochemistry, and neurobiology...

  3. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...is ideal for tracking large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D... time. 1D barcodes can be purchased from many companies such as Corning. Tubes can even have specific ...specific barcodes etched or printed onto them by companies such as Tubewriter. Any lab can benefit from using...

  4. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target DNA ...other enzymes such as Cre-recombinase drastically expanding the base-editing reporter toolbox. The Dow lab...Serebrenik AA, Shaban NM, Brown WL, Harris RS (2019) A panel of eGFP reporters for single base editing by APOBEC-Cas9...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...thousands or even millions of base pairs. Several companies have worked on this technology, but Oxford Nanopore...time, but does not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences...identity and length, but disregards the specified discrepancies. Any sequences that are a perfect match from...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...NGS) technology. As our repository continues to expand, we realized the need to modernize our approach... number of deposited plasmids increased, the accompanying sequencing data collection and analysis eventually...Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython.  We started at the usual...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...and Pandey et al., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S....antibodies can be tethered on the viral surface, expanding the range of targetable cell types. DIRECTED is...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Nucleocapsid Protein antibodies #211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against... against vinculin as a loading control (right panel). Image from Addgene. These antibodies are recombinant...

  9. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine...

  11. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian cells...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded...sequence between two attR/L sites. Image reused from Pandey et al. 2024 under CC-BY 4.0 license.   An, ...doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ....  How do you find validated antibodies? Many companies that produce and distribute antibodies validate...through their publications or as a review on a company website. It is important to review the data and...can help you compare antibodies from different companies, such as Validated Antibody Database and CiteAb...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...labeling compared to immunoprecipitation. The top panel depicts the identification of heat-shock proteins...in HCT116 cells (colorectal cancer). The bottom panel depicts the identification of general heat-shock...different interneuron populations. The enhancers span all four major classes of GABAergic interneurons...

  16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9...https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B (2015) Streamlined...

  17. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...by the trial. During clinical trials, expansion of participants based on early results can enhance the...treatment regimens were not chosen carefully. Participants in clinical trials should be picked based on...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...allowing users to easily isolate and subsequently expand monoclonal cultures.  Interpreting flow cytometry...experimental sample. In addition, you should include a panel of “fluorescence-minus one” controls, samples stained...experiment except one. The fluorescence-minus one panel will help you set up gating during data analysis...

  19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting...

  20. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... large sequences (up to 10 kb) into the genome (Pandey et al., 2024; Yarnall et al., 2023) Prime editing...doi.org/10.1038/s41587-021-01025-z Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph...410. https://doi.org/10.1038/s41587-021-01039-7 Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...
Showing: 201 - 220 of 538 results