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We narrowed to 651 results for: PPL

Showing: 201 - 220 of 651 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... plasmid or group of plasmids' functions and applications. We hope that these articles make it easier ...FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power of mCyFP1, the Lin... Coselection efficiency is so high that some applications may permit working with edited “pools” of cells... subtilis has a fully sequenced genome, many applications in biotech, and is considered the most studied...regulation to genome engineering that enables new applications, such as reversing the effects of dCas9 binding...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...requiring people who are willing to quickly learn, and apply their knowledge to a variety of problems. In this...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients manage...literature, and critical analyses skills were applicable to my cases. I can directly use my understanding...the gap between the science and the commercial applications of a particular therapy. With your scientific...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...you. One such option is the CRISPR Design tool supplied by the Zhang lab. This simple website invites ...the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...you can order them yourself through whatever DNA supply company you currently use. You can also find many...selected gRNA’s, and has the bonus benefit of supplying you with primers for validating your edit in mice...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ... Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage tracing in other systems...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving... Currently, Brainbow techniques are also being applied to study model organisms such as Drosophila and...brain and other biological systems. Interested in applying Brainbow to your research? Check out Brainbow ...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... closely read the publication, including the supplemental data. You’re most likely to find thorough characterization...spectral and kinetic activation information, in the supplemental data instead of in the main text.  Then, check...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve learned about...and Tol2), each of which has several different applications (mutagenesis screens, transgenic animals, gene...Garcia-Perez J (2010) DNA Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/...Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome...

  9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...

  10. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...

  11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...RNAs can also be purchased from various other suppliers with a typical turnaround time of 5-7 days. The...reagents could be purchased directly from commercial suppliers without investing time and labour on reagent preparation...gene-specific crRNAs and matched ssODNs from commercial suppliers of CRISPR reagents (turnaround time 5-7 days);...optimizing CRISPR/Cas9 genome-editing methods and applying them to study brain tumour biology. When not pipetting...

  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

  15. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

  16. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...regions in the near future. Additional Resources Apply to Recieve Equipment through Seeding Labs' Instrumental...

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...antibodies. This is the most usual level of evidence supplied to support the suitability of an antibody for ...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife...

  18. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...for both transient and stable gene expression applications in plants. This smaller, easily customizable...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...only for plant engineering and biotechnology applications, but also for foundational studies of promoter...

  19. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...making them a promising tool for cell therapy applications. Dual base editors What about combining the ...editors with increased activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https:.... (2017). Improving the DNA specificity and applicability of base editing through protein engineering ...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...
Showing: 201 - 220 of 651 results