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We narrowed to 536 results for: SHI

Showing: 201 - 220 of 536 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ... 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...?) As a result, I’d always end up stressed and rushing, no matter how hard I tried to build in extra time...especially on days when I was tired, stressed, or rushing. I would spend the time quietly running through... consider it this way: When you’re stressed or rushing, you’re much more likely to make a mistake. When...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...mCyRFP1 include a redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence...s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon... Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with further development, could ...

  5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    .... PubMed Central PMCID: PMC4517063. 3. Mizushima, N., Yoshimori, T., & Levine, B. (2010). Methods in Mammalian...Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the...

  6. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory, or clinical...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

  7. 27 Hot Plasmids from 2016

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    Blog Post
    ...plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...likelihood of creating a functional knock-out via frameshift and minimize off-target effects. The library ...Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles... et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...

  10. Design Tips for Prime Editing

    Type
    Blog Post
    ...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are ...Wang et. al., 2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin ...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...and Challenges CUREs inspire students to take ownership of their work and become more actively involved...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings...

  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...employees. The teambuilding culture helps us build relationships and thereby work better together.I realize I...encourage everyone to make an effort to build relationships with their colleagues. It can be easy to get...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage...

  19. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...as we obtain more genomic data about plasmids (Shintani et al., 2015). Now, bioinformatics tools help ...10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial....2015.00242 Pubmed PMID: 25873913 Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility...

  20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...coffee breaks. While this was not the more intense mentorship experience that happened at ASM Microbe, we connected...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...
Showing: 201 - 220 of 536 results