We narrowed to 232 results for: SPL;

1. March for Science

   Type

   Blog Post

   ... that thoughts on some non-political issues are split along party lines (18). Feeling like an apple will...

2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...mutational scanning: ligand binding assays, phage display assays, ubiquitination activity assays, toxin activity...

3. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ... heterogeneous cell mixes, like bulk lympho-/splenocytes, you might only be interested in one of the various...

4. Tips for Getting a Faculty Position

   Type

   Blog Post

   ...experts ask you about the details and then you can display your deep knowledge of your experiments and field...

5. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   ...digest products of the unmodified plasmid will be split into two smaller bands (Figure B, digest A). In ...

6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   ...state. In contrast, Brainbow-3.1 and -3.2 do not display default fluorescence due to the addition of translation-blocking...

7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   ...previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend...

8. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   ...choose are teaching the stuff you want to know and display character traits and skills that you hope to grow...

9. Transferable Skills Guide: Teamwork

   Type

   Blog Post

   ...slowly gone crazy. As it was, my partner Mike and I split the work, shared the credit, and we both won accolades...

10. Antibodies 101: Validation

    Type

    Blog Post

    ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

11. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    ... Types of transposons Transposable elements are split into two classes based on their mechanism of transposition...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ...D.A., et al., Generation, purification and transplantation of photoreceptors derived from human induced...

13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    ...critical role the work would not have been possible. Display of work in artistic exhibitions or showcases (not...

14. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    ...when transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary...

15. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    ... to tumor formation in iPSC-derived and iPSC-transplanted mice. Cre-deletable or Tet-inducible lentiviruses...

16. Protocol - Over-Agar Antibiotic Plating

    Type

    Protocol

    ...Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin stock...

17. Weighing Reagents Protocol

    Type

    Protocol

    ...provide more accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing...

18. Water Bath Protocol

    Type

    Protocol

    ...bath, but will likely involve a dial or a digital display you can set to the correct temperature. Set up ...

19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    ...cassette. The simpler system of pCRISPomyces-2 displays a higher editing efficiency, perhaps due to its...

20. General Transfection

    Type

    Protocol

    ...obtaining high levels of virus. HEK293T cells should be split 3 times a week: Monday: Plate 1x10 6 cells in a ...