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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 11 results for: SPL;

Showing: 1 - 11 of 11 results

  1. Pipetting Protocol

    Type
    Protocol
    ... expel liquid into the pipette tip, the volume display which shows the set volume for the the pipette,...section will help you understand how to read each display to dispense the correct amount of liquid reagent...), while 0.5 µL would read as 050. Each volume display on these pipettes will also have small tick marks...you will be rotating dials behind the numerical display or the top of the plunger of the pipette. Select...

  2. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ... of PEG solution to each 100 mL of supernatant. Split into 2 x 500 mL sterile bottles as needed. Add stir...cells were imaged with a 10X objective and were split 2 days before these images were taken. Last reviewed...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...provide more accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing...

  6. Water Bath Protocol

    Type
    Protocol
    ...bath, but will likely involve a dial or a digital display you can set to the correct temperature. Set up ...

  7. General Transfection

    Type
    Protocol
    ...obtaining high levels of virus. HEK293T cells should be split 3 times a week: Monday: Plate 1x10 6 cells in a ...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ...correctly assembled plasmids by designing primers to split an antibiotic resistance gene to effectively create...

  9. Lentivirus Production

    Type
    Protocol
    ...obtaining high viral titer. 293T cells should be split 3 times a week: Monday: Plate 1×10 6 cells in a ...

  11. CRISPR Library Amplification

    Type
    Protocol
    ... is better than pulling motion Take care not to split or gouge agar during the scraping process. Add each...
Showing: 1 - 11 of 11 results