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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 466 results for: Sele

Showing: 201 - 220 of 466 results

  1. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...                     A post shared by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore...

  7. Plan Your Experiment

    Type
    Collection
    ...To: Plan Your Experiment Overview Select Genetic Manipulation Select Expression System Design gRNA Publications...mammalian cell lines Select Your Target Sequence and Design Your gRNA Once you have selected your CRISPR components...where the edit should occur and select a target sequence nearby. Select gRNAs based on predicted on-target...that are available for your particular experiment. Select Your Desired Genetic Manipulation Different genetic...manipulations require different CRISPR components. Selecting a specific genetic manipulation can be a good ... exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need ...promoter for Cas enzyme and gRNA Presence of a selectable marker (drug or fluorophore) Delivery method ...

  13. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base ...

  14. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Cas9 system to developing an antibiotic that selectively kills only the bacteria you want it to, leaving...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids ...directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase... RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically ...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted...heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... you can find or select for only the cells that received the plasmid. Selectable Marker...plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian...curated by species, epitope tags/fusion protein, selectable marker, viral expression, and more. Plasmid...cloning site (MCS), an inducible promoter, and/or a selectable marker. They are frequently used in molecular...many elements to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most...Genome Modification, mRNA Regulation, and More Selectable Markers Species-Specific Expression If you want...retroviral gene expression with GFP or mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For...
Showing: 201 - 220 of 466 results