We narrowed to 249 results for: TIR

1. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ... et al. (2016). To date, small probes that are entirely genetically encoded do not exist, but the development...

2. The Fluorescent Vegetables in Aptamer Soup

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   ...Fluorescence is observed when these RNAs complex and the entire aptamer can hybridize and fold. (Alam et al., 2017...

3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...basics about CRISPR in our CRISPR guide Browse our entire COVID-19 plasmid collection ...

4. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also...

5. 10 Steps to a Perfect Science Talk

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   ...does follow the general structure and flow of the entire session. I myself have a terrible habit of saying...

6. Site Directed Mutagenesis by PCR

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   ... mutation) in a PCR protocol that amplifies the entire plasmid template. The parent template is removed...

7. Evolution of Lab Techniques

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   ...sequencing technologies could one day sequence an entire genome for under $1,000 (10). The future of sequencing...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma...

9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   ...solutions that could save both your lab and the entire research enterprise time and funds. It’s time to...

10. CRISPR 101: Off-Target Effects

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    ...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...

11. Viral Vectors 101: Optogenetic Tools

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    ... photoswitches OptoXRs are great for activating entire signaling pathways, but what if you’re interested...

12. Plasmids 101: Dimers and Multimers

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    ... on as a dimer, depicted on the right, with the entire monomeric sequence duplicated in tandem, resulting...

13. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ...real-time correspondence via e-mail throughout the entire year. In addition to their direct training role...

14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...gene editing process. See below for details. The entire process from start to finish took about 1 month...

15. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...trivializes downstream characterization, as the entire culture can be harvested instead of microscopic...

16. A Guide to Getting Started in Undergrad Research

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    ...industry, so feel free to explore! Addgene has an entire page dedicated to different career options from...

17. Transferable Skills Guide: Teamwork

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    ...more things necessary to make a successful team. Entire books have been written about this! But this is...

18. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...2) if the dsDNA was short enough, a ssDNA whose entire complementary strand was degraded. Beta: Beta protects...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...Shuttle device; 5 days after the transfection, the entire plate was scanned for V5 tagging using an Operetta...

20. A Needle in a Base-Stack: Cas9 Structural Biology

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    ...the recognition domains—including the CTD and the entire REC lobe—do not structurally resemble any other...