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We narrowed to 948 results for: ache

Showing: 201 - 220 of 948 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... spectrophotometry. Achieving the highest-quality DNA requires taking care at each step, but some purification... use one of two common approaches — columns or magnetic beads. Both approaches involve binding the plasmid.... DNA quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep... the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed in a column...usually by centrifugation or vacuum. In a bead-based approach, you mix specially coated magnetic beads into ...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In these... Column Mini, Midi $$ No No Not mentioned No Macherey-Nagel Beads, column, plate Mini, midi, maxi, mega...

  2. Technical Design of a Western Blot

    Type
    Blog Post
    ...significantly from lot-to-lot, and have to be re-validated each time a new lot is purchased. Monoclonal antibodies... these mixtures are defined by the manufacturer, each rAb in a mixture can be individually validated. ‌...primary antibody with the new reporter. Finally, each new conjugate will have to be tested to ensure it...therefore cannot compare Bis and Tris gels or blots to each other. Once you know your gel type, you’ll need ...amount of optimization that can be done.  Note that each type of transfer requires different equipment. Wet... long exposure times (up to thirty minutes!) to reach the limit of detection. Film also needs to be digitized...require no consumables, produce digital images, reach their limit of detection quickly (~1 minute), and...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ... thanks for our guest blogger, Gairik Sachdeva! Gairik Sachdeva is a Ph.D. in Bioengineering and works...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, ...challenges, quickly breaking them up into smaller achievable tasks, and then executing on them in a timely...creative solutions to emerge. They enjoy the fact that each new challenge will require knowledge of new topics...situations, and personalities. Teams striving to achieve a lot of great output in a short amount of time... and to help manage that, most consulting firms coach and counsel employees on the different personality...core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance providers...

  4. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t throw...New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping...lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways to further... throw away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...tips. This is part of a cultural change in how we approach waste that needs to be supported at all levels...industrial and residential markets. Rethinking our approach to lab waste is something we all need to do. Remember...

  5. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...’s very sad because it’s years of work,” Sacher says. Sacher hopes that as Phage Directory expands, it...When Jessica Sacher, a microbiologist from the University of Alberta, saw that scientists were using ...recognize a specific part on the bacterium making each phage is very specific. Some infect only one or ...really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step forward...Since the inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand their role...information and potential for successful phage therapy, Sacher and Zheng also saw the need to archive phage reagents...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...and flexibility to the CRISPR editing world. The approach, originally published in Nature, is a “search-... sequence location The prime editor extends the reach of CRISPR genome editing, as it can edit near to...prime editing typically needs to be optimized for each application, and the efficacy varies widely — it...versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...strand (Chen et al., 2021). The team applied this approach to both the PE2 and PE3 systems, naming the new...optimized enzyme can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc...improved efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for short...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...   Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...You can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...generate the strictly monomeric EGFP variant, mEGFP (Zacharias et al., 2002). GFP needs oxygen Chromophore formation...protein module that can report on location. This approach enables the study of proteins in cells, but not...tolerated in my fusion It is standard practice to attach the GFP to the N- or C-terminus of another protein...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...live across the country or around the world from each other are collaborating on projects. When I was ...experiments can get done and data analyzed between each meeting, but short enough that everyone stays on...meet. You and your collaborators should then hold each other accountable to these expectations once they...checking in about them at your recurring meetings and reaching out if any milestones are missed. Spend time in...think about having collaborators spend time with each other in person. One possibility is to do a lab ...lab exchange, where researchers spent time in each other’s labs. This is an opportunity for the researchers...researchers involved to learn techniques from each other, access specialized equipment, or ensure that techniques...

  9. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...intellectual journey. Practical approaches One approach to achieve this goal could involve participating...moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking field ...or visiting museums can be excellent methods to detach from the constant stream of thoughts related to...predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace serendipity....” This quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters...

  10. Pipetting Protocol

    Type
    Protocol
    ... Although each pipette performs the same function, the numbers are read differently on each type of pipette...to 100 µL P200 20 to 200 µL P1000 100 to 1,000 µL Each pipette should be used with the appropriate tips...This section will help you understand how to read each display to dispense the correct amount of liquid... picture above), while 0.5 µL would read as 050. Each volume display on these pipettes will also have ...to expel excess liquid from the tip once you’ve reached step 8. Continuing to hold down the plunger, gently...liquid so that it's easier for the pipette tip to reach the liquid. Aim to let only the pipette tip into...

  11. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches...Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine,... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...you can better design your donor DNA template to achieve more efficient genome editing - all influenced ...

  12. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...more progress you will make in reaching them. Of course, you won’t reach all the goals you set, but the...bring more than their knowledge and teaching to help a Mentee reach goals. One of the most important contributions...relationships, so that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit... adjust expectations, and record milestones in reaching goals. The more time and thought you put into ...being a Mentor: Learn other perspectives Develop coaching skills Encourage your creative thinking Enhance...

  13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Repeat for each QuickSeal tube. (Optional) For first time users, it is a good idea to assay each fraction...of 60% iodixanol and 45 μL of phenol red Underlay each solution into a QuickSeal tube in the order below...mL fractions in microcentrifuge tubes. Repeat for each QuickSeal tube. The first 3 mL collected corresponds...below the 60–40% interface with an 18 ga needle attached to a 10 mL syringe. The bevel of the needle should...note that iodixanol is not easily removed. After each spin, add more formulation buffer and sample and...stain. Note the increased number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M...

  14. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...with greater spatial and temporal control than is achieved by traditional pharmacologic methods. A number...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...secondary signaling pathways with light. This is achieved by coupling endogenous signaling molecules with...the activation curves (Marshel et al., 2019) for each tool, and you may have to test and optimize the ...sources used to prevent activation of both tools with each light source used.   Channel and activation kinetics...it is important to understand the details of how each tool performs and the consequences of off-target...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ... of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...leadership, and inappropriate tools or writing approaches can lead to frustration, delay of publication... someone could raise their hands or signal the approach of a brain break, where everyone stands up, disappears...general chatter will be allowed with a different host each time that moderates conversations. This can help...social distancing. The take home message is the approaches/tools we have to foster creative collaboration...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    ... the topic at hand. Invite one “ambassador” from each affected team to be the representative in the meeting...standing team meetings, provide agenda sections for each person to add a bullet or two about what they are...many points as they like to the written agenda, but each only gets to address one point aloud. This keeps...separate section at the top of the agenda, and assign each one using group members’ initials. If appropriate...Consider whether you actually need a full hour to achieve the purpose of the meeting (see “defining the scope... and are able to provide more timely feedback on each other’s projects. For all-company meetings, monthly...still works best. Figure out what works for you for each of your meeting types (see above). We’ve provided...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    ...overview). Until recently, the approach has been hampered by the need to tag each protein of interest and, ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and...provides researchers better flexibility and makes the approach more accessible, as it reduces the need to generate... barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains...multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent protein...four different fluorescent proteins, and inserting each visual barcode into its own subclone, they were ...

  18. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves...some of the people and organizations who helped us reach this milestone. Viral service supporters We would...from laboratories all over the world. With this reach and exposure in the community, we saw the opportunity...shipping systems and protocols for viral vectors to reach scientists around the world. Our Software Engineering...our systems for this new type of material. The Outreach Team is getting the word out about this new resource...preps derived from plasmids in the repository. The Outreach Team has been making sure scientists know about...

  19. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...revolutionary physical approaches. However, with each new technical achievement, the complexity of the...disciplines. In biology, innovative new imaging approaches are constantly being developed which combine ...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation... greatly increases accessibility. Through this approach, the Planktoscope, a portable device for studying...and Deployment." bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable...

  20. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... any eligibility provision, don’t guess. Simply reach out to the program coordinator with your question...best to draft a cover letter that is customized for each internship. Lastly, have your resume and cover letter...statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle...opportunity for themselves. This is ultimately the wrong approach. It is very likely you know individuals in your...cohort. This may sound counterintuitive since that approach seems to create competition for your own spot.... Pasteur These tips are a set of common sense approaches that will prepare you for the competitive landscape...Hospital of Braintree. Rose is passionate about outreach among low-income communities to educate parents...
Showing: 201 - 220 of 948 results