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We narrowed to 230 results for: crispr c plasmids

Showing: 201 - 220 of 230 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...Addgene's CRISPR Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and Resources...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...like CRISPR-Cas9 are not only valuable but often essential. Find a collection of CRISPR plasmids that ...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...Biology Guide CRISPR Guide Bacteria are commonly used to create, store, and replicate plasmids of all types...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...

  2. Zhang Lab CRISPR Page

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    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery...

  3. TALEN Expression Vectors

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    Collection
    ...Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into ...2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287 JDS71 HD RVD C 32288 JDS74...with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome... genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our Empty gRNA...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...

  5. Neurodegeneration Research Collection

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    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...fragments. This system has been used to construct plasmids for use in model organisms such as E. coli and...

  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post
    ...others at the Sainsbury are working on a lot of CRISPR tools for editing plant genomes. We will definitely...Quick, Versatile Plant Transgenesis with GreenGate Plasmids  ...assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011...

  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Turquoise (CeNL; Plasmid #85199), Yellow (YeNL; Plasmid #85201), Orange (OeNL; plasmid #85202) and Red ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178) for use in the system outlined in Schmid-Burgk...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions of...

  9. CRISPR References and Information

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    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...Description Plasmids in protocol Download protocol Addgene CRISPR pooled library amplification CRISPR pooled... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...

  10. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...Chemogenetics Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids...Tumor Suppressor Gene CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources ...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test...

  12. TALEN Plasmids and Kits

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    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...acids after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain... TALE plasmids. Genome...

  13. TALEN Guide

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    Collection
    ...Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in...Guide to TALEN technology and using TALEN plasmids TALENs... 2011, the groups describe a set of customized plasmids that can be used with the recently developed Golden...ordered fashion in a single reaction.” Using these plasmids, which are available as a kit from Addgene , custom... TAL effectors could become a key tool in your plasmid toolbox. Addgene has rapidly become an important...Center (www.taleffectors.com) . Whether you work in a C. elegans lab and have been struggling to mutate a ..., Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic...

  14. Luciferase Plasmid Collection

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    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid and the effects...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to monitor ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid...

  15. TALEN Engineering

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    Collection
    ... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and...has yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish (...

  16. Allen Institute for Cell Science Plasmid Collection

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    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

  17. Botman-Teusink Yeast FP Collection

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    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs ...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter...promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2...

  18. Synthetic Biology - Overview

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    Collection
    ...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...components. Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic...

  19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...researcher can flank fluorescent proteins in a plasmid with loxP sites such that their expression is turned... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent ...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role...

  20. CRISPR Guide

    Type
    Guide
    ...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...miRNAs to restrict CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....
Showing: 201 - 220 of 230 results