We narrowed to 622 results for: erf

...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple but effective... sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing of our plasmid samples...

...T7 promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product... and infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka...contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice using a small...

...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...activity. In summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

...Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of the restriction... common chemically-competent cells. You should perform, at minimum, two transformations after a ligation... 101 Posts Resources on Addgene.org Find the Perfect Empty Vector for Your Cloning Experiments Browse...

...the Volume on the Pipette Although each pipette performs the same function, the numbers are read differently...

...and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping into this...me, the Veterans Health Administration was the perfect position because my science policy ‘why’ was to...this question may change over time and that’s perfectly normal!  Action Items:  Start a career journal...

...what I think are the main tips for each step of performing and analyzing a gene edit using Addgene’s lentiviral...lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell extracts from stable pools of A549...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

...phenotypes. These approaches may become even more powerful following a report that one can use linear repair...non-linked loci; pha-1(e2123) mutant worms are perfectly viable at 15 ºC, yet exhibit complete embryonic...recombination repair pathway. Reagents required to perform pha-1 co-conversion are available through Addgene...

... D E Sample 1 Sample 4 F E Sample 2 Sample 5 F Perform data analysis using the instrument’s software. ...

...technique if you are working with any weird and wonderful organisms. While your samples are sterilizing ...

...homologous recombination has not occurred. This powerful negative selection system ensures high editing...Transcriptional repression (CRISPRi) in bacteria As RNA interference does not function in bacteria, most efforts ...inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic Acids Res 44:e143–e143 . https...

...Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration....

...Follow this protocol to perform RNA extraction without a kit....

...“addgene” into the GIF search bar to find that perfect response GIF.     We have GIFs for every...via GIPHY   If you’re also looking for that perfect GIF for your TikTok or Instagram story, we also...

...long the scientist waited after injection before performing the experiment. Every entry has an accompanying... so that you can see for yourself how the AAV performed in that experimental setup. For example, this ...

...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

...up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This wonderful piece...

...on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ when Dr. Prakash... By connecting art and science, a fascinating interface is created that produces loads of inspiration ...

...validity of your biological model and the technical performance of your assay. But, in many discovery-oriented...each with 4 gRNAs per kinase, so screens can be performed with either 4 or 8 gRNAs per gene. Further, the...

...reagents) along with information critical for the performance of a reagent, e.g., antigen retrieval conditions...Knowledge-Base is an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX ...