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We narrowed to 636 results for: erf

Showing: 201 - 220 of 636 results

  1. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...support your professional development I had a wonderfully supportive mentor and graduate office. I cannot...ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering, I was worried...Scientists Read Emma Markham's Post on Finding Your Perfect Job After University Browse All of Our Career Posts...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    ...contamination could interfere with interpreting the results of transfection assays. Go forth and perform your DNA...when the kits buffers ran out. Homemade buffers performed the same as those provided with the kit. Although...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some time now. When...readout of your samples.  Of course, if qPCR were perfect there would be no need for ddPCR. One issue with...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

  4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...the genome. Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help... the techniques from Table 1, provides us with powerful GPS-like techniques to explore dark matter in ...interests span machine learning, proteomics, and user interfaces for math.     Additional Resources Beyond the...

  6. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...methods can interfere with binding, and some common antibody buffer components can interfere with conjugates... non-overlapping epitopes so that they do not interfere with each other. The detection antibody may either...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...Addgene’s repository) and make it easy to find your perfect match. Choosing a Cas9 for your knockout experiment...deaminase to where the edit is desired, and it performs the programmed edit within the editing window ...with this tool is that the desired edit will be performed on all relevant bases within the editing window...

  8. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...Wild-type AAV2 has been reported to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner...requires an electron microscope, it is not routinely performed on all new viral vector preparations. Addgene ...PMCID: PMC3706409. 7. Zeltner, Nadja, et al. "Near-perfect infectivity of wild-type AAV as benchmark for infectivity...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...you're becoming an expert in your field and are the perfect person to be writing the review! Doing so is a ...life’s work: so remember that done is better than perfect. Good luck! References 1. Haery, Leila, Ryan C...

  10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...cannot be ignored. They have the potential to interfere with the function of resident ER chaperones and...and retained in the ER. FPs have been undeniably powerful and successful tools that have enabled numerous..., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments ...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... to the consistency of the reagents and hands performing the experiment, not the reproducibility or consistency...independent aliquots of cells and plasmids, be performed on different days, etc, but outside constraints...incorporating additional positive and negative controls, performing multiple replicates, and ultimately getting interpretable...

  12. General Transfection

    Type
    Protocol
    ... (am): Observe fluorescence, harvest cells, or perform your experiment Equipment Class II, Type A2 Biological...

  14. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow for antibodies to ...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...analysis is performed yielding a graph of edits in the input population or clone.   How to perform a TIDE...homology-directed repair events, or maybe you’re performing a single base pair edit with a prime editor. ...

  16. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... virus directly using saliva without having to perform RNA extraction but that may work only if there ... Motif) for target cleavage. This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK...fluorescently labelled substrate, gets activated and performs non-specific collateral cleavage. This cleavage...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... GFP is such a well-validated, all-round good performing probe, it is the first choice when selecting ...reduces its size. The substantial size of GFP may interfere with the activity or the localization of the fusion...C-terminus of another protein. This may, however, interfere with the function of the protein. This is clearly...

  18. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...repeating for each target. Alternatively, you can perform simultaneous detection with a mixture of primary...steps, in order to ensure your antibodies are performing their best and that the sample is not being degraded... P., & Yong, W. H. (2019). An Introduction to Performing Immunofluorescence Staining. In W. H. Yong (Ed...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...Running undigested DNA on a gel as a control when performing a diagnostic digest is a simple but effective... sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing of our plasmid samples...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...T7 promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product... and infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka...contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice using a small...
Showing: 201 - 220 of 636 results