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  1. Lentiviral Guide

    Type
    Guide
    ...: Tet- or Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids available...system through the addition of a chimeric 5' LTR fused to a heterologous promoter on the transfer plasmid...Wild type Hybrid: 5'LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...
  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...
  3. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... References Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to...
  4. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...
  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer...
  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further...the sequence of your gene and of the fusion protein. Tag or Fusion Protein Common uses Representative...vector backbones curated by species, epitope tags/fusion protein, selectable marker, viral expression, and... by: Species-specific expression Epitope tag or fusion protein Selectable markers Viral expression and... vectors - Inducible expression, fusion proteins, and more Sandmeyer Lab...mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning of ...the function of your favorite gene. For example, fusing your protein to an epitope tag, such as Flag...
  7. Bacterial Expression Systems

    Type
    Collection
    ...between the RNAP subunit α fusion protein in pBRα-β flap and the λCI fusion protein in pACλCI-β-flap results...interest are fused to AraC or a dominant negative AraC. Preferential association of AraC-fusion homodimers... expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease ...fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors with N and C-terminal fluorescent...that can be used to create fluorescent protein fusions with your protein of interest. See an overview ... of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid. pZE31...
  8. The MTA at Addgene

    Type
    Blog Post
    ....  Why the MTA?  The MTA process can be a bit confusing, and it’s certainly frustrating to find out your...
Showing: 201 - 220 of 362 results