We narrowed to 268 results for: gnal

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   ...particularly useful in generating transient edits of signaling molecules (RNA) as opposed to permanent edits ...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...circuits with many guide RNAs connected because the signal degrades at each layer. If we could fix these things...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   ...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...

4. Hot Plasmids February 2024

   Type

   Blog Post

   ... to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing...

5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   ...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a ...

6. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...small molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular...

7. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   ... function and result in a ubiquitous background signal that makes it hard to visualize the precise location...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   ...outspoken in their opposition to unions, potentially signaling a rough road ahead for students interested in ...

9. Plasmids 101: Protein Expression

   Type

   Blog Post

   ...University and is particularly interested in cell signaling and communication. She loves being able to help...

10. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    ...delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https:...

11. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    ...expressed and purified. Although a ~1% change in signal might not make a very powerful tool, it was promising...

12. Antibodies 101: Flow Compensation

    Type

    Blog Post

    ...telling your cytometer to adjust the fluorescent signals by a specific value between the two channels. It...

13. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ...Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification RANbodies: Reporter Nanobody...

14. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...

15. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ... Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells...

16. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    ....0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ...

17. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...typically invisible information into actionable signals. Let's say I wanted to detect every primary metabolite...

18. COVID-19 Resources

    Type

    Collection

    ... route for SARS-CoV-2 infection to host cells . Signal transduction and targeted therapy, 5 (1), 283. ...

19. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ...Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...

20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...Marvin E., et al. "A protein-tagging system for signal amplification in gene expression and fluorescence...