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We narrowed to 477 results for: mag

Showing: 201 - 220 of 477 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...looks like the one found on the left side of the image below. It has a promoter (blue arrow) followed by... using UV light – please note that UV light can damage DNA and that proper personal protective equipment... worn when visualizing using UV as it can cause damage to the eyes and skin. When running a gel for purification...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... have expressed GFP) that I couldn’t get a good image. You could probably also do Western blotting against..., but to do it you would need a pretty good gel image. If you are analyzing short DNA fragments, run PAGE...Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing Gels ...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...contain many epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always...concentration of cell lysate was added to each well. Image from Sun et al., 2010.   Enzyme-linked immunosorbent...fluorophore-conjugated secondary antibody or Fab. Image from Harden et al., 2014.   In vivo uses For ...

  4. Centrifugation

    Type
    Protocol
    ...they are distributed evenly around the center (see image to right). Even distribution ensures that the centrifuge...Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the user. If...different protocols. Improper use of a centrifuge can damage the equipment, your samples, or you, so be sure...

  5. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...

  6. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...  HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository From ... Knowledge-Base  Any IBEX multiplex tissue imaging Data repository See blog post mAb3D Atlas...

  7. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...addgene.org to learn more. A great submission includes: Images that clearly show viral spread and cellular tropism... a functional vector such as a channelrhodopsin Images showing the injection site as well as the target...

  8. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...let us know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. Caulobacter ... Figure 2: The components of Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic...

  10. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...highlights written as 8 tweetable facts. Note: The images used in this post were created using data from ...scientific domains tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure ...

  11. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG...for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...source of mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al..../fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics...

  15. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...sample were loaded in wells B01-H01. As shown in the image (Figure 1) and table below, the concentration of... to the lower dilutions (blue). As shown in the image (Figure 2) below, the concentration of RPP30 positive...

  16. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...includes frequently cited antibodies for use in brain imaging (Andrews et al., 2019), validated in both western...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... of producing fluorescent proteins and enzymes. Image from Moore et al., 2021.   This TX-TL system... genes known or suspected to be involved in DNA damage response and DNA repair.  A new CRISPR pooled library...

  20. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons)....maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled...
Showing: 201 - 220 of 477 results