...lens which magnifies the image, then the ocular lens, where the image is further magnified before it finally...10x magnification. To determine the final magnification of your image, multiply the magnification of your...now) is the magnification power, such as 4x, 10x, or 20x. A 4x objective magnifies the image four times...properties of a microscope are magnification (the ability to make an image larger) and resolution (the ability...your eyes. These lenses determine the magnification of the image and the resolution your microscope can...10x objective magnifies it 10 times, and so on. The ocular lens also provides magnification and the power...also 10x, you would actually be seeing a 100x magnified image of that object! Equipment Light Microscope ...

...Metal spatula Razor blade Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant...the sink. Take a brightfield image of the gel with an appropriate imaging system. Recombinant antibody... QC and would not be used. Section 3: Image Analysis Use ImageJ or a similar photo software to determine...bands to the overall lane. Import the gel image into ImageJ. Select File . Select Open . Choose the location...location of the file to open. Change the image type to 8-bit . Select Image . Select Type . Choose 8-bit . Determine... using ready-to-use biosafe Coomassie stain and ImageJ software. Protocols...250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated by denaturing...

...These cells were imaged with a 10X objective and were split 2 days before these images were taken.... Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

...growth. Left panels: bright field images; right panels: GFP channel images.... Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning 10...Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers ...capsid proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., ...

...they are distributed evenly around the center (see image to right). Even distribution ensures that the centrifuge...Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the user. If...different protocols. Improper use of a centrifuge can damage the equipment, your samples, or you, so be sure...

...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...sample were loaded in wells B01-H01. As shown in the image (Figure 1) and table below, the concentration of... to the lower dilutions (blue). As shown in the image (Figure 2) below, the concentration of RPP30 positive...

...nearest sample. Note: To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...

...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

...solution and then rinse it in water before you can image the gel. Pour the agarose into a gel tray with the...expose for as little time as possible to minimize damage to the DNA. Note: When using UV light, protect ...

...Device Roller Spatula Platform shaker Cold room Gel imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g...Cover the membrane with clear plastic and use a gel imager with chemiluminescence detection or a dark room...

...temperature, as prolonged storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous...

...glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive...

...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

...cause the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce...

...Heat-inactivated FBS Polybrene PBS pH 7.4 without calcium or magnesium (cations can affect the attachment of adherent...

...pipette with the appropriate dispense volume: This image shows the P2, P10, P20, P100, P200, and P1000 as...

...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

...that will bind to and replicate it. The following image shows the ends of the ORF and how these are used...

...Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...