We narrowed to 565 results for: mme

1. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...experiment achieving the gRNA representation recommended in the publications associated with these libraries...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

2. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

   ...launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in 2017. These travel awards are...this year’s Michael Davidson and Roger Tsien Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger...

3. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   Blog Post

   ...Apoptosis or “programmed cell death" plays a pivotal role in an array of biological processes including...PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology 35.4 (2007...

4. Viral Vectors 101: Inverted Terminal Repeats

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   ... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...through ITR sequences. GC tails are often located immediately outside of the ITRs in the plasmid backbone and...

5. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

   ... the project we are focusing on what we call “asymmetric collaborations.” These are transient partnerships...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...

6. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ...reagents, several journals or organizations now recommend or require authors to use specific PID formats...Addgene_87155)” Please note that we only provide recommendations for how to cite using the PIDs in the material...

7. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ... this is less of a concern, but Addgene still recommends plating the transformation mixture on solid media...interest about library use, let us know in the comments! Additional Resources on the Addgene Blog Check...

8. R Bodies: Membrane-Rupturing Microscopic Tools

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   Blog Post

   ...promoter results in high R-body expression  (Schrallhammer et al 2012). This made them an appealing target...3759909. PubMed Central PMCID: PMC213417. 2. Schrallhammer, M. et al. Tracing the role of R-bodies in the...

9. Advice for Scientists Starting a Lab

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   Blog Post

   ... that future hires will look up to." She also recommends to seek out advice from seasoned PIs who can ...it is once it gets fully up to speed.” He also comments that it is also surprising “ how easily people...

10. Improved Plasmid Maps Powered by SnapGene

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    Blog Post

    ...section. Analyze your plasmid sequence For more immediate, high-level analysis, you can click directly on...us know what you think about the updates in the comments section below and keep checking in at blog.addgene.org...

11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ...plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain...samples that are requested by scientists, will immediately be screened by our QC scientists to confirm the...

12. Clear and Accessible Writing

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    ...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt...people.  Whether you start small with alt text or immediately dive into applying these four steps elsewhere...

13. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...a variety of stakeholders from industrial and commercial operators, government agencies, active researchers...scientific innovation amongst school children through summer workshops and interactive discussions. Not only...

14. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post

    ...guest blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it ...your research or career? Share your story in the comments below!   Additional resources on the Addgene...

15. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...engineering is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the...impact repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which...

16. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...terminus. With that new design in place, Konermann immediately saw an order of magnitude improvement in activation...pooled libraries in your experiments, Addgene recommends that you review the steps for how to use pooled...

17. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice...turns a stranger into a friendly colleague. Recommended Reading & More: Scientist Networking: What is...

18. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ...particularly for applied projects intended for commercialization. Many of the genetic pieces developed over...able to overcome them. Share your stories in the comments below! And if you have developed a genetic tool...

19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ...complementary skill sets, and “talk a good game." Mike recommends that the team should initially include a visionary... an idea or product? Please let us know in the comments section! And read on for more great information...

20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post

    ...resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain is ccdB Survival...these resources further. Feel free to offer your comments and suggestions, and even to submit a guest blog...