...mutations. Moreover, introducing new DNA seems impossible with current tools, thus quieting some fears ...in cases of disease prevention. However, it is important to consider that already-tested CRISPR alternatives...clinical applications could be considered. It is important to consider the question of broader applicability... large step forward in the CRISPR field, it’s important to remember that the study hasn’t achieved any...

...transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...degradation In the Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest... chimeras For each protein-of-interest, it is important to determine which terminus can accommodate the...

... how these structures are generated, why it’s important for cells to sense their formation, and how clinicians...in turn lead to genome instability. Thus, it’s important to strike a balance between productive levels ...be delivered or anticipating this response are important considerations when using these reagents. RNA...stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease...

...Nature has evolved ways to prevent degradation of important cellular RNA molecules with post translational...Frontiers in Genome Editing). This is particularly important in experiments in which viability is being compromised...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase...recognition site. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and ...and researchers have adapted the components for use in genome manipulation. Cre recombinase, originally...

...project is the best approach to a given problem, Empowers the writer, And offers insight into a career ...understand the RFA. The RFA contains all of the important information required for a successful grant application... to determine the exact value of costs. It is important to be aware of supply costs per year, how many...Grant applications have a standard “Our work will empower the world” tone that differs from publication writing...

...conclusions. Being vigilant about these errors is an important part of maintaining the quality of reagents, especially... especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are ...tags, promoters, and other critical functional components. Fun fact! Some older plasmids in our collection...each of their plasmids, from descriptions of key components of the plasmids to partial, full, or annotated...

... grant early! First off, and perhaps the most important bit of advice I can suggest, is to give yourself...Originality is essential for grant success The two most important things reviewers will be looking for in your research...from accounts or HR on the judging panel. It’s important for this person to get a clear and concise explanation...going to be an element of luck involved so it’s important not to throw in the towel if 1 or 2 funding bodies...

...transcribed but do not encode proteins. They play important roles in many cellular and regulatory processes...into the break sites. Knock-in experiments are important for visualizing ncRNA localization and function...complex and not yet fully understood, so it is important to carefully select the target locations of knock-in...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...

...assembly. Simply put, it assembles individual DNA components into larger units or circuits with functional...Transcription units (TUs), which are functional units composed of multiple parts transcribed together. They can... test a variety of different orientations and components within the system to maximize production potential...multiplexed gRNA systems as well as additional components such as selectable markers, promoters, enhancers...

...making wide-scale gene activation seem next to impossible. The development of CRISPR/Cas systems, however...rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, SAM, and VPR... fusion protein, rather than relying on a two-component system dependent on gRNA design (SAM) or peptide...lines (Sajwan et al., 2019). SPH SPH combines components of SAM and SunTag activators, fusing the epitope...

...One such control is dividing up the essential components of a virus into different plasmids. For a retroviral... are not encoded in the transfer plasmid, the component that physically integrates into the host genome... Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.    Viral... would be infected by a lab-grown virus, it's important to understand the risks of the specific virus ...

... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     ...Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung epithelial...). In their search, they found mucins have an important role in viral entry, which are inhibitors of SARS-CoV...research through sharing reagents has never been so important.     References and resources References ...

... sections as a primary research article, it’s important to understand that presenting your work in poster...certain posters resonate with you (or not). It is important to reflect on these impressions when thinking ...concise as possible, communicating only the most important results to your audience. With a poster, you have...particular styles recognized to work well. It is important that your readers can navigate your poster and...

...to make sure that everyone can be informed and empowered to make their own decisions about genetics, both...their academic environment and attend seminars, symposia, or courses in social, health or policy-related...either giving or running these events will be important both in preparing you for future work in these...communicate your work to a non-technical audience is an important skill for every scientist. After all, scientific...

... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent ...et al., 2024). Each plasmid expresses all the components of the biosensor in optimal proportions from ...Waldron We recently added two antibodies against important targets for neurodegeneration research: Anti-MAPT...

...better at producing a compound of interest, thus allowing them to optimize the compound production process...Although it is possible to make a timer system composed of two separate FPs (one that matures quickly ...Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009): 1863-1868...

...individual libraries can vary significantly, it’s important that we have an amplification protocol developed...and the desired coverage for the library (How important is it that every single plasmid in your library...protocol. Created with BioRender.com.   The most important factor to consider is your desired library coverage...used for libraries that are similar in size and composition as examples. Table 1 provides an overview of ...

...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged... the system is that it requires an additional component to be engineered or expressed beyond the tag and...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...

...detector using a series of mirrors and filters. Importantly, conventional flow cytometry uses one detector... flow cytometry for a given experiment, it is important to consider how many parameters you want to use...conventional and spectral flow cytometry, it is important to consider the expression levels of the markers...two highly overlapping cellular markers, it is important to pair them with two fluorophores that have distinct...