We narrowed to 292 results for: nif

1. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   ...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...

2. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ... limited understanding of plant biology is a significant technical obstacle to plant synthetic biology...

3. Antibodies 101: Secondary Antibodies

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   ...which means that indirect methods are often significantly longer than direct ones.  Even though the secondary...

4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...

5. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ... Jennifer Tsang...

6. RaPID Detection of RNA-protein Interactions

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   ...datasets. Applications of RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help...

7. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the...

8. RANbodies: Reporter Nanobody Fusions

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   ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

9. Human Germline Editing Using CRISPR

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   ...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

10. Antibodies 101: Conjugation

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    Blog Post

    ...sensitive detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...

12. Addgene's Tips for Plasmid Quality Control

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    ...always be derived from a single colony to ensure uniformity of the culture. Sequencing directly from the ...

13. Career Coaching for Scientists: Why and Where Do I Find One?

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    ... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...

14. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

17. Adenoviral Vector Production and Troubleshooting

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    ...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...

18. Hot Plasmids and Viral Preps - January 2021

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    ...doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

19. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...

20. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...of a lac operon dropped to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly...