We narrowed to 285 results for: nif

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...

3. Antibodies 101: Secondary Antibodies

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   ...which means that indirect methods are often significantly longer than direct ones.  Even though the secondary...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ... Jennifer Tsang...

5. RaPID Detection of RNA-protein Interactions

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   ...datasets. Applications of RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help...

6. Antibodies 101: Conjugation

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   ...sensitive detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

7. RANbodies: Reporter Nanobody Fusions

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   ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

8. Human Germline Editing Using CRISPR

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   ...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

9. Addgene's Tips for Plasmid Quality Control

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   ...always be derived from a single colony to ensure uniformity of the culture. Sequencing directly from the ...

10. Career Coaching for Scientists: Why and Where Do I Find One?

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    ... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...

11. 5 Steps to Writing Grant Proposals for Grad Students

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    ...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...

12. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

13. Hot Plasmids and Viral Preps - January 2021

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    ...doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ... May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving...

15. Viral Vectors 101: Calcium Sensors

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    ...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...

16. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...of a lac operon dropped to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly...

17. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

18. Adenoviral Vector Production and Troubleshooting

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    ...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...

19. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...interested parties can do research. This interest manifested itself in the creation of Hackerspaces and Makerspaces...

20. Of Myc and Men

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    ... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...