We narrowed to 291 results for: nif

1. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   ... limited understanding of plant biology is a significant technical obstacle to plant synthetic biology...

2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...

3. Antibodies 101: Secondary Antibodies

   Type

   Blog Post

   ...which means that indirect methods are often significantly longer than direct ones.  Even though the secondary...

4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   ...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...

5. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the...

6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   ... Jennifer Tsang...

7. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   ...datasets. Applications of RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help...

8. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

9. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

10. Antibodies 101: Conjugation

    Type

    Blog Post

    ...sensitive detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...

11. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...always be derived from a single colony to ensure uniformity of the culture. Sequencing directly from the ...

12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    ... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...

13. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...

14. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    ...doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    ... May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving...

16. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...

17. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...of a lac operon dropped to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly...

18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

19. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    ...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

20. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...