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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 562 results for: pac

Showing: 201 - 220 of 562 results

  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested...original plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will ...integration events. In addition, promoter choice can also impact transgene expression. The most accurate method ...

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...TCCGGGATCCGGGCTGCGgctgctgttcctcctgCGCCCTGCGGAGCGGCGA Impact homolog (mouse) TAL3462 & TAL3463 TGGACAAATTAGAGGATCatgacgataataatctGCAGTCTCAGATAGAGGA...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...will likely be spending a few hours on your feet. Pack water and snacks to keep you energized throughout...session, bring extra clips to hold up your poster, and pack a few business cards to tack up next to your poster...hearing the highlights so that you can appreciate the impact of the project as a whole. In addition to time ...

  5. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings. A picture of a 60 mm dish...the plates that do not appear to be yielding well-spaced clones. For rapidly growing cells we sometimes ...the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences) to...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...are sharing stories of our repository’s positive impact on science and scientists around the globe. Our...CRISPR plasmids to labs in ~100 countries.  The impact Addgene’s CRISPR collection contained 197 plasmids...citations.  CRISPR is a story that centers around the impact of science, but our data makes it clear that it...

  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...committees, and how the culture of the university impacts the number of women reaching senior positions. ... those amazing students that want to have a big impact on the world and tell them to work in plant science...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site. Four proteins...INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations...INTEGRATE system follows a simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo...

  10. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...have done the hard work for you by immunizing an alpaca, isolating HCab antibodies from its serum, and ...for generating nanobodies are outlined below; no alpaca required. Decide Which Nanobody To Use: The table...

  11. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

  12. An Introduction to Adenovirus

    Type
    Blog Post
    ..., including the E1 region). The space in the viral particle’s capacity that is made available by this ...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...

  15. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...focus. Find yourself a block of time and a quiet space where you can address the issue head on. Prepare...26 ideas, over half of which, when implemented, impacted the entire company. It is important to note that...team has used these techniques to optimize our lab space, our protocols and even our Lab Inventory Management...

  16. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...routinely use Nanopore technology to sequence our packaged viral DNA for quality control. This gives us a...the issue and resolve the assembly. Keeping up the pace Our QC team constantly faces the challenge of keeping... backend, has enabled the QC to keep up with the pace of deposits while maintaining quality. Maintaining...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...  Here's what you'll find in this post:  New compact, efficient prime editors A versatile and precise...tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna Stroik The ...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983–4002...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive ...Transfection of the DNA pooled library and appropriate packaging and envelope plasmids into HEK cells allows production...and composition of your library can drastically impact what conditions it needs to properly amplify.  ...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...resources: ImpactStory, a website that helps researchers go beyond citation rates to measure the impact of their...Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in ...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...
Showing: 201 - 220 of 562 results