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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 201 - 220 of 538 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence is only...Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. coli genome...loci to generate CRISPR RNAs (crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to...material, which must also contain a species-specific sequence known as a protospacer adjacent motif (PAM). The...CRISPR array contains many unique protospacer sequences that have homology to foreign DNA. Protospacers...Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the...genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova et ...

  2. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where...noise, reducing the read depth necessary when sequencing and lowering the amount of tissue needed per ...subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using...

  3. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the tranposon to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion...between a greater number of extinct transposon sequences and then testing various combinations of changes...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Export Sequence mScarlet-I Dorus Gadella 85061 pmScarlet-H_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-H...Palmitoylation sequence from GAP43 EGFP Connie Cepko 61099 Lck-GFP Membrane Palmitoylation sequence from Lck... KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL sequence mScarlet-I...2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3...mCherry-Mito-7 Mitochondria Mitochondrial targeting sequence(COX8A) mCherry* Michael Davidson 37354 pQC NLS...36206 pmTurquoise2-NES Non-nucleus Nuclear Export Sequence mTurquoise2 Dorus Gadella 85062 pmScarlet-i_NES_C1...85060 pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Dorus Gadella 37351 pQC membrane TdTomato...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...semi-synthetic or entirely synthetic VL and VH sequences. Phage display also allows for screening scFv ...antibodies. To do this, antibody variable domain sequences are expressed as scFvs and screened with in vitro...assays to select for strong binders. Then the sequences for the variable domains of the selected scFvs...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...The raw data is then normalized to a reference sequence present in the same amount in all samples (usually...to run multiplex assays, allowing the reference sequence and the gene of interest to be amplified in the...for both the gene of interest and the reference sequence. At the end of the run, each droplet is read as...Complexity of the gene of interest Some gene sequences are harder to amplify and quantify than others...

  7. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ... generating insertions or deletions in the DNA sequence. This same approach is effective to target short...very small deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed...Cas9 generates double-strand breaks, a new DNA sequence can be knocked-in, or introduced, into the break...off-target effects since promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...target site sequence (21-22bp) and need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have... - Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended .... Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return...phosphorylated oligos designed based on the target site sequence (20bp). CRISPR-Cas9 Cre expression vectors for...phosphorylated oligos designed based on the target site sequence (20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre...

  11. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...sgRNA) that bears complementarity to a 20bp target sequence (Qi et al. 2013). Modifying the CRISPRi system... will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional regulator...Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... a genetic sequence and integrate it into any DNA sequence containing the 38-50 bp sequence recognized...they optimized the specific attachment sites via sequence engineering, enabling even more efficient integration...

  13. Viral Production at Addgene

    Type
    Blog Post
    ...please contact us. Full sequencing of the Viral Genome Next-generation sequencing is performed on viral ...genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol gradient...genomes isolated from the final AAV preparation. Sequencing results are analyzed to confirm the identity ...

  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... effective, ivTRT may be more challenging for sequences with complex secondary structure. Today, multiple.... Miura et al. (2018) suggest using a 50-80 bp sequence around the desired insertion site to search for...C-terminal insertion with Easi-CRISPR. Potential guide sequences (arrows) and their PAM (bold highlighted text)... knock-in design may naturally remove the gRNA sequence or place it far from the PAM; if not, you can ...guide. This design also separates the gRNA target sequences from their PAMs, preventing re-cutting after editing...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF. Negative...protein is present, but cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein... (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In the presence...binds to promoters containing putative operator sequences (CuO) (linked in groups of 6), allowing transcription...which activate their respective UAS and lexAop sequences but are also regulated by QS and quinic acid. ...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... a ~20 nucleotide targeting sequence and ~75 nucleotide scaffold sequence) is consistent between plants...contain a plant pol III promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos...is commonly tagged with a nuclear localization sequence to enhance targeting to the nucleus, and several...the specifics - promoters used, precise protein sequences or domains, and methods of delivery - the underlying...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used ...plant source), by tuning the length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies...
Showing: 201 - 220 of 538 results