We narrowed to 526 results for: sequence

...eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from viruses, but ...leading to separation between the end of the 2A sequence and the next peptide downstream.4 The "cleavage...experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G D V E E N P ...of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (...Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (Hum Gene Ther. 2009 Aug;20(8...

...walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie... generate C->T or G->A transitions in a target sequence in live cells. This technique uses catalytically...a deaminase to make the transitions at target sequences specified by gRNAs. Caveats, potential specificity...

...by an RNA guide (gRNA) that harbors targeting sequences. Browse our collection of empty gRNA plasmids ...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used...

...assessment, sterility testing, and viral genome sequencing....Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing for your chosen plasmid...

...without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these...these requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the...Adler et al.   You can also search by protein sequence with BLAST, using the homepage search bar. Once... other parameters, such as E-value for protein sequence searches. Additionally, the Phylogeny tool allows...

...presence of any specific RNA sequence. In the absence of the target RNA sequence (in this case, norovirus ...strains and even other viruses by modifying the sequence of the toehold switch. For example, Green has ...proteins using peptide-phage display and deep sequencing, revealing that they bind to a much larger subset...

... as a guide to complementary RNA sequence. Once a compatible sequence is found (typically RNA that the...purposes similar to that of vaccines. If you know the sequence of a virus, you could introduce some of its dsRNA...due to their abundance of R-loops and repetitive sequence. All of the R-loops described above play distinct...productive levels of R-loops and putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple...sensing systems fail, diseases and steep cellular consequences arise. dsRNA An immune response is activated...Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). ...

...protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the...called pTet) have been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most...or other species by codon optimization of their sequence and eliminating interactions with endogenous factors...Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional...

...easy enough to contemplate DNA in terms of its sequencing, in vivo studies should always take DNA’s chromatin...expression pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts...of auxin, this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged...(minimal 44 amino acids auxin inducible degron sequence) tagged proteins. Degradation of your protein ...

...Yamagata et al used previously published nanobody sequences that targeted the following antigens: GFP, RFP...actin-binding protein gelsolin, but any nanobody sequence could be used to make a RANbody. RANbodies are...secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture...useful RANbody, consider leaving a link to its sequence in the comments below. References Sanes, J.R....

...with a 1.9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More...libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-...backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used...

...whole exome sequencing of embryo derived-ES cells.They also conducted whole genome sequencing in selected...rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing sites in the...off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos. Since...

...adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards... We’re also building a portal to easily share sequences and characterization data for over 150,000 plasmids...

...containing the modifying sequence flanked by approximately 50 base pairs of sequence homologous to the chromosomal... typically contain both a protein modification sequence and a selectable gene product for isolation of...

... reaction for traditional restriction cloning. Sequence and Ligation Independent Cloning (SLIC) could ...Invitrogen and based on lambda phage integration sequences, relies on site-specific recombination reactions...components and define their final order through sequence homology. Similarly, Golden Gate assembly allows...FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR cloning method." ...

... the exact original sequence, it can restore the original protein-coding sequence using a recoding strategy...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs and possibly cas9...unpredictable ecological effects. Because the consequences of such a mistake would necessarily extend far...

...fluorescence, like in the pMuSIC library, or by sequencing the unique DNA barcodes. Figure 1: Schematic...libraries accounts for chromatin, position, and sequence features in the selection of sgRNAs. Other CRISPR...library consists of broad host range regulatory sequences ready to be assembled with other elements using...

...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where...noise, reducing the read depth necessary when sequencing and lowering the amount of tissue needed per ...subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using...

...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the tranposon to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion...between a greater number of extinct transposon sequences and then testing various combinations of changes...

...?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...semi-synthetic or entirely synthetic VL and VH sequences. Phage display also allows for screening scFv ...antibodies. To do this, antibody variable domain sequences are expressed as scFvs and screened with in vitro...assays to select for strong binders. Then the sequences for the variable domains of the selected scFvs...