We narrowed to 265 results for: ttl

1. Antibodies 101: Reading a Flow Plot

   Type

   Blog Post

   ...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ...

2. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   ...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ...

3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   ... Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues...

4. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ... I enjoy doing code reviews because it’s like a little peek into the mind of the developer and I get to...

5. How to Write a Scientific Review Article

   Type

   Blog Post

   ...real time as I write. Things are going to get a little crazy (meaning you are probably going to cite hundreds...

6. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   ...with compounds similar to those they have been battling for centuries. As such, resistant populations ...

7. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   ...scientific journals flies under the radar and gains little notice. A 2009 research study found that 12% of...

8. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   ...as well. Inhibition of neuronal activation is a little more complicated since basal neuronal activity ...

9. Data Freedom: The Expansion of Data Sharing in Research Publications

   Type

   Blog Post

   ...searching and extraction of information. Looking a little further ahead, journals may move towards encouraging...

10. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...etc) require unique promoters and there is very little crossover. Generally speaking, promoters in bacteria...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ... ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction: Fragmented Genome...

12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    ...principles of gating, you’ll first need to learn a little bit of theoretical background on the different ...

13. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm, but keep ...

14. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    ...(the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great option...

15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    ...you can create custom RNA binding proteins in as little as 3 days. Abil et al., 2014 show that you can ...

16. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    ...learn new things, so rehash common knowledge as little as possible. Don’t use a lot of jargon in your ...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...evade immune detection because they produce very little or no viral protein. To solve this problem, two...

18. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    ...joining (NHEJ) pathway. It’s estimated that very little dystrophin correction (about 4%) is needed to see...

19. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    ...work! But let’s take a moment to recognize your little prokaryotic minions that carried out the labor-...

20. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    ...importance in design – an optimized sequence will do little if it is in the wrong place, but because the target...