Antibodies 101: Reading a Flow Plot
Type
Blog Post
...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ...
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Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...partitioning (Schumacher, 2012). This concept is a little more complex than simply competing for replication... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... I enjoy doing code reviews because it’s like a little peek into the mind of the developer and I get to... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...scientific field. Reaching out to people who have little in common with you is much harder than starting... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues... -
How to Write a Scientific Review Article
TypeBlog Post...real time as I write. Things are going to get a little crazy (meaning you are probably going to cite hundreds... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...with compounds similar to those they have been battling for centuries. As such, resistant populations ... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...scientific journals flies under the radar and gains little notice. A 2009 research study found that 12% of... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...as well. Inhibition of neuronal activation is a little more complicated since basal neuronal activity ... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...searching and extraction of information. Looking a little further ahead, journals may move towards encouraging... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...etc) require unique promoters and there is very little crossover. Generally speaking, promoters in bacteria... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post... ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction: Fragmented Genome... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...(the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great option... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm, but keep ... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...principles of gating, you’ll first need to learn a little bit of theoretical background on the different ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...you can create custom RNA binding proteins in as little as 3 days. Abil et al., 2014 show that you can ... -
10 Steps to a Perfect Science Talk
TypeBlog Post...learn new things, so rehash common knowledge as little as possible. Don’t use a lot of jargon in your ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...evade immune detection because they produce very little or no viral protein. To solve this problem, two... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...joining (NHEJ) pathway. It’s estimated that very little dystrophin correction (about 4%) is needed to see...