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We narrowed to 185 results for: INV-E

Showing: 111 - 120 of 185 results
  1. Summer SciComm: Preprints

    Type
    Blog Post
    .../2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel Z (2019) On the...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...
  2. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...allow students to propagate the plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ori and...answer questions about the evolution of the genes involved in methionine synthesis (5). The budding yeast...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...
  3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and in vivo...approaches.  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...
  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...expansion.  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A...in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule is...
  5. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a ...blog post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, ...origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...
  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression...a popular tool in the field as it provides a noninvasive method for manipulating cell activity, especially...provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your...specific circuits. Methods for targeting expression involve tissue specific promoters, AAV serotypes, and the...
  7. Of Myc and Men

    Type
    Blog Post
    ...(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10 displays...Pelham’s first published peptide tagging experiment involved a peptide from the neuropeptide substance P. This...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions and sticking...
  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880 Kwan...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...
  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...
Showing: 111 - 120 of 185 results