...genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and in vivo...approaches.  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...

...expansion.  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A...in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule is...

...Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a ...blog post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, ...origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...

...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...Siouve, E., Becavin, C., Depardieu, F., & Bikard, D. (2018). Genome-wide CRISPR-dCas9 screens in E. coli...e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S.,...

...Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression...a popular tool in the field as it provides a noninvasive method for manipulating cell activity, especially...provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your...specific circuits. Methods for targeting expression involve tissue specific promoters, AAV serotypes, and the...

...(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10 displays...Pelham’s first published peptide tagging experiment involved a peptide from the neuropeptide substance P. This...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions and sticking...

...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880 Kwan...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...

...doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...things at Addgene, each part of this new process involves a significant amount of QC. First, we perform ...

...Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...