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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27 results for: INV-E

Showing: 1 - 20 of 27 results

  1. CRISPR Guide

    Type
    Collection
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...Siouve, E., Becavin, C., Depardieu, F., & Bikard, D. (2018). Genome-wide CRISPR-dCas9 screens in E. coli...e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S.,...

  2. Bacterial Expression Systems

    Type
    Collection
    ...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein... to purify it. Specific bacterial strains such as E. coli BL21(DE3) are used as biofactories to express...cytoplasmic and periplasmic protein expression in E. coli , Bacillus subtilis , and Pichia pastoris . ... (reconstructed) BiFC Dan Mulvihill 39866 pWA PAS-E BAD K-GAFm iRFP (reconstructed) BiFC Vladislav Verkhusha...reliably drive expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene ...twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible Promoters Repressible...Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others)...Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease... Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM...9. PMID: 26355004 Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, ...as a powerful defensive strategy against viral invaders, and this system is proving to be just as useful...CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy the phages upon re-...

  4. Mammalian RNAi Tools

    Type
    Collection
    ...Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs..., M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions...Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin... silencing mechanism. It is a useful tool to investigate the roles of specific genes or to knockdown genes...

  5. Arf GTPase Family

    Type
    Collection
    ... (Link opens in a new window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright...Santy, L. C., Schürmann, A., Wilhelmi, I., Yohe, M. E., & Kahn, R. A. (2019). ARF GTPases and their GEFs...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...referred to as a minimal triad, with each component involved in GTPase signaling activity. Figure 1: Locations...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Goodson, N. B., Park, K. U., Schwanke, M., Office, E., Schneider, S. R., Abraham, J., Hensley, A., Jones...CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...aide in their studies of the regulatory networks involved in retinal development the lab utilizes a CRISPR...

  7. COVID-19 Resources

    Type
    Collection
    ...2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...infection, such as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and SARS-CoV-2. Find ...protease that primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme...superfamily, binds to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L -...infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV... BSG - Wang, K et al, 2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein...

  9. CRISPR References and Information

    Type
    Collection
    ...the Jin-Soo Kim Lab . CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation (Link opens in...repression and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm...John Doench , David Root , and Hyongbum Kim Labs. E-CRISPR (Link opens in a new window) Identifies gRNA...Currently supports over 20 model and non-model invertebrate species. Developed by the Kate O'Connor-Giles...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta 5 FLJ76359, HLA-DRB HLA-E major histocompatibility complex, class I, E DKFZp686P19218, EA1.2, EA2.1...subfamily 2, group E, member 1 TLL, TLX, XTLL NR2E3 nuclear receptor subfamily 2, group E, member 3 ESCS,...PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa EP1 PTGER2 prostaglandin E receptor 2 (subtype ... HLA-JY3, HLC-C, PSORS1 HLA-E major histocompatibility complex, class I, E DKFZp686P19218, EA1.2, EA2.1...MGC9284 CTSB cathepsin B APPS, CPSB CTSE cathepsin E CATE CTSL1 cathepsin L1 CATL, CTSL, FLJ31037, MEP ...cell lectin-like receptor subfamily C, member 3 NKG2-E, NKG2E KLRC4 killer cell lectin-like receptor subfamily...IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin heavy variable...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial ...TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal His and NusA fusions...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...BiFC vector (pEarleyGate201-YN) Zeocin® is an InvivoGen trademark. Return to top Content last reviewed...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase gene allow for increased copy number in E. coli and expression of luciferase in the cytoplasm...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha...212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha...

  13. Chemogenetics Plasmids

    Type
    Collection
    ...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs, including DREADD constructs...

  14. Plan Your Experiment

    Type
    Collection
    ...Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh...Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J. S., Friedman, N., & Regev, A. (2016...10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna, R. E., & Doench, J. G. (2020). Design and analysis of CRISPR–Cas...methods is RNA delivery of Cas and gRNA. This method involves using in vitro transcription reactions to generate...

  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction (from 3.2.1 - 3.2.2...a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate onto LB agar plates with the...2007. PubMed . Transformation of plasmid DNA into E. coli using the heat shock method. Froger...CRISPRs into Cells of Interest NOTE: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation...

  16. Adenovirus Plasmids

    Type
    Collection
    ...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...produced by several methods, the most common of which involves homologous recombination of adenovirus plasmids...

  17. Promega Plasmid Collection

    Type
    Collection
    ... for various protein expression systems including E. coli , mammalian cells, and cell-free systems. HiBiT...real-time monitoring of signaling pathways, investigating RNA regulation, tracking protein degradation...

  18. Cre-lox system

    Type
    Collection
    ...intron, preventing Cre expression when cloning in E. coli . This alteration enables the generation of ...construct (unmodified Cre will cause recombination in E. coli , deleting the floxed portion of a construct...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...below shows the three types of rearrangements: inversion, deletion, and translocation. Figure 1. Recombination...opposite directions, recombination results in an inversion and the region of DNA between the target sites...binding to these sites can result in excision, inversion or translocation depending on the orientation ...

  19. Ras Pathway

    Type
    Collection
    ...this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource...variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. ...Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1...

  20. Neurodegeneration Research Collection

    Type
    Collection
    ...factors. For example, variations of Apolipoprotein E (APOE), such as the ε4 allele, are a risk factor for...symptoms through medication and surgery. PD primarily involves the malfunction and death of dopamine-producing...other large scale studies. Researchers are now investigating the role that these additional genes may play...
Showing: 1 - 20 of 27 results