...Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target... variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can make ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...finding. Transcriptional program changes from this screen successfully differentiated cells resembling all...from Joung et al.)    Putting MORF to work Screens and selections In its current format, the MORF ...library is most immediately useful for TF library screens. Previously engineered TF libraries were not as...

...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ...within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation...on the expression of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...editors for your research Read our CRISPR guide Find plasmids for your research ...David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying...

...Addgene Blog Plasmids 101: Common Lab E. coli Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden ...on the F sex factor plasmid of E. coli, is part of a toxin-antitoxin system encoded by the ccd operon, ...-containing vectors in mind, any plasmid that contains the F plasmid (F’ strains) will also be resistant..., which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein ...Cells that lose ccdA through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure...disrupted allowing cells expressing the recombinant plasmid to propagate. Any cells that contained non-recombinant... of clones that do not contain the recombinant plasmid and therefore makes the cloning process much more...

...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ... 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions that are...selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection targets...collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage indicator (...precise plant breeding. Find dicot prime editing plasmids here! Vu, T. V., Nguyen, N. T., Kim, J., Song... proteins for mammalian cell expression. These plasmids will be useful not only for future studies of ...proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid, ...

...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for...factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA takes up ....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques....2014.04.022. Epub 2014 May 15. PubMed.  Find the plasmids from this publication at Addgene Dimeric CRISPR...nbt.2908. Epub 2014 Apr 25. PubMed.. Find the plasmids from this publication at Addgene ...mutations through homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ... Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...researchers custom design their own engineering plasmid tools in about a week. Taking advantage of Golden...

...About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest Plasmid Tools for the ...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...of conditions. They are composed of 4 proteins encoded in a single operon and were first expressed in ...produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild type and mutant...acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime... Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent AAV1 vectors and are suitable for extended...found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled ...