...restriction digest! Then, you can transform or transfect the cells that you want to use for your experiments...

...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...

...Virus Name Description Link to Video General Transfection Introduce plasmid DNA to mammalian cells Lentivirus...Produce lentivirus with a polyethyenimine (PEI) transfection protocol Fluorescence Titering Assay for Lentivirus...preparation Antibodies Name Description Link to Video Transfection Introduce plasmid DNA to mammalian cells to ...

... protein expression observed using transient transfection approaches, generating cell lines using lentiviral... stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it eliminates...variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers...

...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...

...facilitates viral entry into the host cell.  Transfecting these three plasmids into cells enables the ...

...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...