We narrowed to 144 results for: transfection

...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...

...preparations. Name Description Link to Video General Transfection Introduce plasmid DNA to mammalian cells Lentivirus...Produce lentivirus with a polyethyenimine (PEI) transfection protocol Fluorescence Titering Assay for Lentivirus...applications. Name Description Link to Video Transfection Introduce plasmid DNA to mammalian cells to ...

... protein expression observed using transient transfection approaches, generating cell lines using lentiviral... stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it eliminates...variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers...

...useful for lineage tracing, evaluating infection/transfection efficiency, selecting desired clones, and making...

...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...

...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

...facilitates viral entry into the host cell.  Transfecting these three plasmids into cells enables the ...

... as early as 48-72 hours post-transformation/transfection in a transient assay. For stable transformation...

...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

...a vector that also expresses mCherry to mark transfected cells. The nanobody recruits the killswitch to...