We narrowed to 469 results for: Lif
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TypeBlog Post... your lab bench, we need to (this is a little simplified): Find the appropriate glycerol stock within...happy lab staff. Barcoded materials make everyone's life easier, the time and capital cost of getting up ...
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Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...drugs were withdrawn from the US market because of life-threatening health effects. Eight of these posed...breast cancers, and 145,000 more quality-adjusted life years. While most of the results were positive, ... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...number of sequencing reactions, you can make your life much easier by selecting the right primer. This ... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...information you need to choose well. What are their qualifications? Do they have certifications? How can they ...posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats, has an outstanding ... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...and function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870. Kaas GA, Zhong C, Eason... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...between cells. Tang et al., eLife 2020. https://doi.org/10.7554/eLife.61080 A new tool-kit for... -
A Look at Addgene's QC Process
TypeBlog Post... utilize an array of tools that rely on DNA amplification and manipulation to produce and edit plasmids...places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender.... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...rare inherited metabolic diseases with severe and life-threatening consequences. They result from defects...on CDG patients and to increase their quality of life by reducing their risks for infection and other ... -
Lab Automation at Addgene
TypeBlog Post...wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this...Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software for Biology Tips... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia...al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed PMID: 27175853. Pubmed ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...with reduced viral titer, but improved transcript life, and so should be handled with care (4). For this... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...changed our whole outlook on the possibilities in life for employment? It never happened – there was never...moment”. But there were a lot of very small steps. Life in a cutting-edge synthetic biology lab Keira and... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that — by developing...Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...Chaokun Li and colleagues. Here you simply PCR-amplify your vector and your insert, DpnI digest away the...1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...or extracellular destination. At the end of their life cycle, proteins may be transported through the vesicles...location of proteins it is also possible to follow the life cycle of viruses and intracellular bacteria – have... -
Of Myc and Men
TypeBlog Post... those questions in this post as we explore… the life and times of the myc tag. Origins of protein tags...would help the field explore Myc’s role in cell proliferation and tumorigenesis. Using two different synthetic...