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We narrowed to 650 results for: CHI;

Showing: 331 - 360 of 650 results

  1. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor...inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human...

  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...

  5. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

  6. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...you volunteer to do science outreach and teach children about science? Or perhaps you have presented in...specifically to assist graduate students in job searching. Start your career planning early, start now Don't...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

  12. Hot Plasmids - February 2022

    Type
    Blog Post
    ...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

  13. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

  15. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

  17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

  22. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  23. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  24. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately...

  26. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

  27. Antibodies 101: Conjugation

    Type
    Blog Post
    ...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  29. Working with Nuclear Receptors

    Type
    Blog Post
    ...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

  30. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...
Showing: 331 - 360 of 650 results