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We narrowed to 509 results for: plasmid dna

Showing: 331 - 360 of 509 results

  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections with ...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright...Effector nucleases are shown bound to the target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...j.stem.2025.01.012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users...delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 ...

  3. DNA Quantification

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information During...your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol. Protocols...spectrophotometer using the solution the DNA is resuspended in, but with no DNA added. 'Blanking' measures the ... Protocols DNA Quantification...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering...important to get a quick and accurate reading of DNA concentration and purity. This can be done with a... maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is at 260nm. Protein...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

  5. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also create... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also... like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration...glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid... and growth temperature, can be found on your plasmid's information page. The next day you will be able...

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing lentiviral...recombination between the transfer and packaging plasmids does exist. To further reduce this possibility...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...uses non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...expression plasmids that have been designed for use in plants. CRISPR...

  9. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your colleagues...Virus-associated DNA Each viral vector service request will also include virus-associated DNA, which is a ...a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...widely cited in publications. All pricing includes DNA amplification, viral vector production, purification...inclusion is what gives you permission to use the plasmid....

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but this ...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

  11. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...interaction due to mismatches between the guide and the DNA target. CFD scores are then modified (“transformed...sequence, one by one, from the PAM outwards so that the DNA sequence is altered but the protein sequence they...software Resources on Addgene.org Find CRISPR plasmids and resources Download the CRISPR eBook Read our...

  12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...reconstituted after integration; and a sequence of stuffer DNA after the gRNA target site. Donor B contains: 400...Since this approach is dependent on double-strand DNA break repair, if non-homologous end joining (NHEJ...2013) Oversized AAV Transductifon Is Mediated via a DNA-PKcs-independent, Rad51C-dependent Repair Pathway...therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out the Addgene...

  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA....genomic changes, researchers use DNA repair templates with homology to the DNA flanking the DSB and a specific...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background ...stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence...which would denature DNA polymerases from other species. In addition to the template DNA and the Taq polymerase...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and... are using the same template DNA for all your reactions, the Template DNA can be added to the master mix...the number of nanomoles of DNA times 10. For example, if your lyophilized DNA is 38.5nm, add 385µl of water...

  16. Lentivirus Production

    Type
    Protocol
    ...Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be 83.4 μL of 1 mg/mL PEI in 416.6 μL of OptiPro...the diluted DNA mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ... repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should ...module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2... TALE plasmids. Genome...

  18. Protocol - How to Streak a Plate

    Type
    Protocol
    ...for DNA purification will minimize the chance of having a mixture of plasmids in your purified DNA. This...Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction If you have... stab culture of bacteria and need to purify plasmid DNA from it, you will want to isolate an individual...single colonies, you can proceed to Recovering Plasmid DNA or use the individual colonies for other experiments...antibiotic. Label the bottom of the plate with the plasmid name and the date. It is also a good idea to add...overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead ... 37 °C. This is often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to...

  19. Video Library

    Type
    Protocol
    ...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...Protocol Purifying DNA from an Agarose Gel Gel purification allows you to isolate and purify DNA fragments based...electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly...Protocol Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction...Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR Generate genomic deletions in mammalian...Transformations We walk you through a standard plasmid transformation protocol and offer some tips and...the standard laboratory procedure for separating DNA by size for visualization and purification. Agarose...

  20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...taken up the plasmid. This can serve as an alternative to antibiotic selection. Plasmids of this type .... Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed...each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split... marking/selection: Expression constructs like plasmids often include GFP as a marker to help identify...nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label pathogenic...techniques in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide... plasmids 101...

  22. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...sequence in pEcgRNA. Next both plasmids are transformed in E. coli with the donor DNA for editing to take place...beneficial for plasmid clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only.... pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed... 13 plasmids with 14 rounds of transformation in 30 days. Figure 2: (A) MUCICAT plasmids for ... original pCas/pTargetF system consists of one plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive...the pTargetF construct and then introduce both plasmids into the cell. The edit in the chromosome can ...the pMB1 replicon, resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF...

  23. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break....Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors ..., creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been ...fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage ...

  24. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...the challenges of DNA distribution to ensure scientists everywhere can access plasmids despite the evolving... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...to worry about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  25. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, ... Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine) pTP955...Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice ...column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: Purified nanobodies...Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M...23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies... on Addgene.org Find more nanobody expression plasmids here Additional Resources Looking for monoclonal...

  26. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...derivatives. Unmodified Cas9 causes double stranded DNA breaks that commonly result in a complete loss of...These mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to... CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find pooled libraries available...viability genes as they are subject to an increase in dsDNA breaks that can cause cell death. Thus, CRISPR inhibition...mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto library containing only 3 sgRNAs...

  27. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...random) integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes...AAV. Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene ...in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...

  28. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...episomal expression. Episomes are circular pieces of DNA that reside outside a host cell’s genome. In this... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...levels of AAV vector integration into CRISPR-induced DNA breaks. Nature Communications, 10(1). https://doi.org...

  29. The Challenges of Cell Culture

    Type
    Blog Post
    ...good that it is used as a forensic technique (aka DNA fingerprinting) that can place suspects at the scene...range of testing, from animal doping to forensics, DNA sequencing to drug testing. You can find Nick on ...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  30. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... external signals such as developmental cues and DNA damage. These signals activate a cascade of caspases...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...
Showing: 331 - 360 of 509 results