We narrowed to 877 results for: HA

...properties of modified histones, a genomic region that has mono-methylated and acetylated histones, but not ...magic and Poisson statistics, each sub-population has, on average, one distinct enhancer disrupted. As ...unhealthy cells. Until recently, the human genome has been full of dark matter: enhancers, lncRNAs, repetitive...

...the State Museum has been the best supporter and most productive partnership CINSO has had. Volunteer! ...provide online. Twitter is an excellent resource that has gained a lot of traction with researchers. It is ...

...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...

...but the introduction of type V Cpf1-driven systems has added another option to the CRISPR toolbox. Cpf1’...frequency of desired HDR edits, but the possibility has not yet been experimentally confirmed. To prevent...adaptation of Cas13 to RNA targeting and RNA editing, has further shown the diversity of CRISPR-based systems...

...plasmid we have at Addgene. Each sample at Addgene has four layers of location which can all be extrapolated...hold up to 96 tubes (see image below). Each plate has numbers and letters to give coordinates, from A1 ...replicate sample. The transformation After the DNA has been received and stored, it is put into E. coli ...

...cleavage with some of these peptides, no consensus has been reached on which peptide works best. Likely ... is easiest to start with a plasmid that already has the multicistronic element and reporter cloned in... unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic...

... Lab technician / Research assistant Typically has a Master's or Bachelor's degree Generally a short-term...talking to people, though, try to be conscious of who has your best interests at heart. A great piece of advice...vulnerable about my struggles and what I don’t know yet has helped me cultivate stronger relationships with my...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career options...

...extraordinary collection of talent, of human knowledge, that has ever been gathered at the White House - with the ... not an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  ... on a leaderless team. Sometimes the leader just has to be the person to say “OK, guys, we need to have...anything out the discussion themselves, then one has to question whether that person should be on the ...

... technique developed in David Liu’s lab in 2019, has added considerably more precision and flexibility... the canonical base editing window, base editing has higher efficiency and fewer indels than prime editing... repair (HDR) stimulated by double strand breaks has been widely used to generate precise changes. However...nicking of the unedited strand only after editing has occurred and reduces indels by 13-fold (Anzalone ... al., 2024; Yarnall et al., 2023) Prime editing has a lot of promise for treating genetic diseases. In...

...techniques and troubleshooting tips. The fourth edition has been fully updated with twelve new sections, including...

...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics...

...beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified...predictors because they are just that: rare. “Nature has many genes where there are not rare codons and many...

...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg. Plasmid Serotype Name...

...on single-cell gene expression patterns. The lab has also used CellTags for tagging two cell populations...libraries at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...

...single-molecule super resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent...fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks (max excitation...

...product packaging) for free! It has to be from their company and it has to be packaging (no coated tissue...

...this crisis has highlighted the importance of access to quality materials and data. Addgene has some exciting...

... power of science to improve the world around us has long been known; ensuring all have access to this...information becomes easier to access by the day, it has never been more crucial to understand just how we...

...the field and terminology that is widely used but has a very specific use-case (definition) in a specific...specific field.  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the ...

...large samples. RUBY-Red But recently, something new has been added to the palette. RUBY is a bright red pigment... University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful imaging. ...

...available, the next best thing is a protein that has been validated for an assay that uses similar sample...Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western because...

...protein that no other protein in the expression system has. This is where affinity tags come in – they are peptides...solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...

...scheme, known as the Baltimore replication classes, has been developed around how they replicate (Figure ..., which retains the protein coat (and envelope), has a “gutted” genome with genes central to the replication...

...Throughout my internship, my interest in scicomm has grown and now it feels like an awesome bionerd hobby...Networking Tips Here And when asked what tips he has for better science communication for non-scientists...

...in Biochemistry with a background in virology who has worked with both animal and plant viruses. Now focusing...compounds with various antimicrobial activities (1). She has developed a fast endophyte screening system and plans...

...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

...overlap extension PCR. Although each type of cloning has its advantages, most scientists still encounter several...science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning...

...Avengers This year Addgene, where my wife Lianna has made her career as Senior Director of Biology, is...pre-clinical development for First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy...

...sciences research. The rare disease research community has certainly noticed: This year, the BeHEARD (Helping...McSweeney (right), a graduate student at Duke who has recently moved to Columbia University with David ...

... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...

...share reagents so freely with the academic community has clearly made a huge impact on this field. Advances...talks, I oscillated between amazement at how much has been done with CRISPR technology in just 2 years ...

...an organization such as COMPASS. Although COMPASS has a special interest in ocean science, they have good...Program at the University of California, Santa Cruz, has been turning scientists into confident and connected...

...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...

...trend in funding and space in academic research labs has more students looking for other options. While graduate... skills for alternative careers. Science Careers has developed a web-based, career-planning platform called...

...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...

...increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed probe sets that...high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly screen buffer conditions for... 200 mM KCl at pH 8.5. This optimization method has been used internally at NEB to screen additional ...additional ligases, conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase....

...CRISPR/Cas editing toolbox for C. elegans Recent work has further improved the efficiency of genome editing... (Farboud et al., 2019). Work from the Mello lab has dramatically improved RNP editing with linear repair...elegans (Ebbing et al., 2017). Because C. elegans has an AT-rich genome, it can be difficult to find an...whereas import of CRISPR technology into C. elegans has been a transformative innovation for our community...

...measured using visible light scatter The optical system has both visible and fluorescent light sources. Visible...autofluorescence from your target cell type or that has arisen from fixation. Also include an isotype control...secondary antibody control in which the primary antibody has been omitted from the staining protocol. This control...gating during data analysis.  We hope that this blog has provided you with an overview of flow cytometry for...

..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...